Strain Name:
STOCK Snx2tm1Mag/Mmnc
Stock Number:
011539-UNC
Citation ID:
RRID:MMRRC_011539-UNC
Other Names:
Sorting nexins 2

Strain Information

Snx2tm1Mag
Name: sorting nexin 2; targeted mutation 1, Terry Magnuson
Synonyms: Snx2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Knockout
Snx2
Name: sorting nexin 2
Synonyms: 0610030A03Rik
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Knockout
NCBI: 67804
VEGA: 18
Homologene: 2332
Genetic Alterations
The coding region of exon 1 and the following splice junction site were replaced with a modified gene casette containing the selectable markers HIS3 and neomycin flanked by loxP sites.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype
    Homozygous: No overt phenotype on its own, but 100% lethal at midgestation on a Snx1-/- background, 40% lethal on a Snx1+/- background, and 100% lethal on a Snx1+/-;Hb58+/- background.

    Heterozygous: No overt phenotype on its own or in combination with mutant Snx1 and/or Hb58 alleles.
    Mammalian Phenotype Terms
    Allelic Composition: Snx2tm1Mag/Snx2tm1Mag (Genetic Background: involves: Swiss Webster )

    MeSH Terms
    • Animals
    • Animals, Newborn
    • Carrier Proteins/genetics
    • Carrier Proteins/physiology
    • Embryo, Mammalian/anatomy & histology
    • Embryo, Mammalian/physiology
    • Embryonic and Fetal Development
    • Endocytosis/physiology
    • Female
    • Fetal Growth Retardation/genetics
    • Fetal Viability
    • Fibroblasts/cytology
    • Fibroblasts/metabolism
    • Gene Targeting
    • Macromolecular Substances
    • Male
    • Mice
    • Mice, Knockout
    • Microscopy, Fluorescence
    • Morphogenesis
    • Phenotype
    • Pregnancy
    • Receptor, Epidermal Growth Factor/genetics
    • Receptor, Epidermal Growth Factor/metabolism
    • Serine Proteinase Inhibitors/genetics
    • Serine Proteinase Inhibitors/physiology
    • Vesicular Transport Proteins
    • Yolk Sac/metabolism
    • Yolk Sac/ultrastructure
    • Biological Transport/physiology
    • Carrier Proteins/metabolism
    • Cell Line
    • Multiprotein Complexes
    • RNA, Messenger/metabolism
    • Sorting Nexins
    • Survival Rate
    • Vesicular Transport Proteins/genetics
    • Vesicular Transport Proteins/metabolism
    • Yolk Sac/cytology
    Strain Development
    The original chimera was mated with a Swiss-Webster female, and other background strains were introduced into the mix due to breeding with other mutants during maintenance of the colony.
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cell Biology
    • Developmental Biology
    Donor
    Terry Magnuson, Ph.D., The University of North Carolina at Chapel Hill School of Medicine
    Primary Reference
    • Schwarz DG, Griffin CT, Schneider EA, Yee D, Magnuson T. Genetic analysis of sorting nexins 1 and 2 reveals a redundant and essential function in mice. Mol Biol Cell. 2002 Oct;13(10):3588-600. (Medline PMID: 12388759)
    • Griffin CT, Trejo J, Magnuson T. Genetic evidence for a mammalian retromer complex containing sorting nexins 1 and 2. Proc Natl Acad Sci U S A. 2005 Oct 18;102(42):15173-7. Epub 2005 Oct 7. (Medline PMID: 16214895)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    011539-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.