Strain Name:
MRL.Cg-Tg(Mt1-Crry)26Quig/Mmucd
Stock Number:
011798-UCD
Citation ID:
RRID:MMRRC_011798-UCD
Other Names:
Crry-Tg on MRL/lpr, MRL.Cg-Tg(Mt1-Crry)26Quig/Mmcd

Strain Information

Mt1
Name: metallothionein 1
Synonyms: MT-I, Mt-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17748
Cr1l
Name: complement C3b/C4b receptor 1 like
Synonyms: mCRY, Crry
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12946
Homologene: 128275
Tg(Mt1-Crry)26Quig
Name: transgene insertion 26, Richard J Quigg
Synonyms: Crry transgene
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The pBluescript portion of the vector was removed by SalI digestion. The 19.4-kb MT-Crry DNA fragment was gel purified and microinjected into the pronuclei of 1- cell fertilized CD-1 oocytes, and transgenic mice were generated using standard techniques.
Genotype Determination
Phenotype
Homozygous: not characterized.

Heterozygous: transgene was propagated by breeding into normal CD-1 mice (Harlan Sprague Dawley, Inc., Indianapolis, IN).Offspring were followed for the transgene by PCR as described above. Because normal mice do not have circulating Crry, confirmation of the presence of the functional transgene came from identifying rsCrry in sera by ELISA.
MeSH Terms
  • Animals
  • Autoimmune Diseases/genetics
  • Autoimmune Diseases/immunology
  • Complement C3/metabolism
  • Complement Inactivator Proteins/biosynthesis
  • Complement Inactivator Proteins/genetics
  • Complement Inactivator Proteins/physiology
  • Immunoglobulin G/metabolism
  • Kidney Glomerulus/metabolism
  • Kidney Glomerulus/pathology
  • Lupus Nephritis/immunology
  • Lupus Nephritis/mortality
  • Lupus Nephritis/pathology
  • Lupus Nephritis/prevention & control
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred MRL lpr
  • Mice, Transgenic
  • Receptors, Complement/biosynthesis
  • Receptors, Complement/genetics
  • Receptors, Complement/physiology
  • Survival Analysis
  • Transgenes/immunology
Strain Development
Crry transgenic mouse on CD1 background was backcrossed into MRL/lpr (MRL/MpJ-Faslpr/J) for 23 generations.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
Donor
Richard Quigg, M.D., University of Chicago Medical Center
Primary Reference
Bao L, Haas M, Boackle SA, Kraus DM, Cunningham PN, Park P, Alexander JJ, Anderson RK, Culhane K, Holers VM, Quigg RJ. Transgenic expression of a soluble complement inhibitor protects against renal disease and promotes survival in MRL/lpr mice. J Immunol. 2002 Apr 1;168(7):3601-7. (Medline PMID: 11907125)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011798-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
011798-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
011798-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.