Strain Name:
B6.129X1-Lcattm1Nsa/Mmmh
Stock Number:
011840-MU
Citation ID:
RRID:MMRRC_011840-MU

Strain Information

Lcattm1Nsa
Name: lecithin cholesterol acyltransferase; targeted mutation 1, Naohiko Sakai
Synonyms: LCAT-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Lcat
Name: lecithin cholesterol acyltransferase
Synonyms: D8Wsu61e
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout
NCBI: 16816
HGNC: HGNC:6522
Homologene: 68042
Genetic Alterations
A neo cassette replaced exons 2-5 of the LCAT gene.
Genotype Determination
ES Cell Line
RW-4 derived from 129X1/SvJ
Phenotype
Homozygous: LCAT activity is 1% of wildtype; total and high density lipoprotein (HDL) cholesterol and apoA-I are significantly reduced.

Heterozygous: LCAT activity is 70% of wildtype; total and high density lipoprotein (HDL) cholesterol are significantly reduced.
Mammalian Phenotype Terms
Allelic Composition: Lcattm1Nsa/Lcattm1Nsa (Genetic Background: B6.Cg-Lcattm1Nsa )

Allelic Composition: Lcattm1Nsa/Lcattm1Nsa (Genetic Background: involves: 129X1/SvJ * C57BL/6J )

Allelic Composition: Lcattm1Nsa/Lcat+ (Genetic Background: B6.Cg-Lcattm1Nsa )

Allelic Composition: Lcattm1Nsa/Lcat+ (Genetic Background: involves: 129X1/SvJ * C57BL/6J )

MeSH Terms
  • Animals
  • Disease Models, Animal
  • Female
  • Gene Deletion
  • Gene Targeting
  • Humans
  • Lecithin Cholesterol Acyltransferase Deficiency/genetics
  • Male
  • Mice
  • Mice, Knockout
  • Arteriosclerosis/enzymology
  • Arteriosclerosis/physiopathology
  • Base Sequence
  • DNA Primers
  • Glomerulosclerosis, Focal Segmental/enzymology
  • Glomerulosclerosis, Focal Segmental/physiopathology
  • Kidney/physiopathology
  • Lipids/blood
  • Lipoproteins/blood
  • Mice, Inbred C57BL
  • Microscopy, Electron
  • Phosphatidylcholine-Sterol O-Acyltransferase/blood
  • Phosphatidylcholine-Sterol O-Acyltransferase/genetics
  • Phosphatidylcholine-Sterol O-Acyltransferase/physiology
  • RNA, Messenger/genetics
Strain Development
Heterozygous F1 LCAT-ko mice were crossed to C57BL/6 and progeny were intercrossed to obtain homozygous knockout mice in a mixed 129/C57BL/6 background. One homozygous knockout mouse in the mixed background was crossed to C57BL/6 and heterozygous offspring were backcrossed to C57BL/6 for another seven generations.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular
  • Metabolism
  • Models for Human Disease
Donor
Silvia Santamarina-Fojo, M.D., Ph.D., National Heart, Lung, and Blood Institute (NHLBI)
Primary Reference
  • Sakai N, Vaisman BL, Koch CA, Hoyt RF Jr, Meyn SM, Talley GD, Paiz JA, Brewer HB Jr, Santamarina-Fojo S. Targeted disruption of the mouse lecithin:cholesterol acyltransferase (LCAT) gene. Generation of a new animal model for human LCAT deficiency. J Biol Chem. 1997 Mar 14;272(11):7506-10. (Medline PMID: 9054454)
  • Lambert G, Sakai N, Vaisman BL, Neufeld EB, Marteyn B, Chan CC, Paigen B, Lupia E, Thomas A, Striker LJ, Blanchette-Mackie J, Csako G, Brady JN, Costello R, Striker GE, Remaley AT, Brewer HB Jr, Santamarina-Fojo S. Analysis of glomerulosclerosis and atherosclerosis in lecithin cholesterol acyltransferase-deficient mice. J Biol Chem. 2001 May 4;276(18):15090-8. Epub 2001 Feb 7. (Medline PMID: 11278414)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011840-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
011840-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.