Strain Detail Sheet

Strain Name    :

STOCK Smarca4tm1Mag/Mmnc

Stock Number :

011869-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Smarca4tm1Mag
Name: targeted mutation 1, Terry Magnuson
Alteration at locus: Knockout
Gene Symbol: Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Chromosome: 9
Alteration at locus: Knockout

Genetic Alterations:
Most of exon 15 and all of exon 16 were deleted and replaced with a promoterless neo cassette. Exon 16 encodes putative helicase motifs I and Ia of the catalytic ATPase domain.

Genotype Determination:

  • Donor's Protocol
  • Center protocol and contact for technical support will be shipped with mice.

ES Cell Line: CT129 derived from STOCK

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Survival to blastocyst stage but failure to implant into uterus.

Heterozygous phenotype: Incomplete penetrance exencephaly and glandular tumors.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Smarca4tm1Mag/Smarca4+
        involves: 129S/Sv * CD-1
  • mortality/aging
    • decreased survivor rate (MGI Ref ID J:66861)
      • heterozygous pups survive at less than the expected 1:2:1 ratio
    • partial postnatal lethality (MGI Ref ID J:66861)
      • some heterozygous pups die immediately after birth
  • nervous system phenotype
    • exencephaly (MGI Ref ID J:66861)
      • exencephaly is observed in some heterozygous embryos (5/36) at E16.5-E18.5
  • tumorigenesis
    • increased tumor incidence (MGI Ref ID J:66861)
      • differentiated epithelial tumors are observed in some 16+ month old mice (3/20)
      • large subcutaneous tumors appear in neck or inguinal regions
Smarca4tm1Mag/Smarca4tm1Mag
        involves: 129S/Sv * CD-1
  • mortality/aging
    • complete embryonic lethality before implantation (MGI Ref ID J:66861)
      • homozygous embryos are not found after E3.5
      • embryos fail to hatch from zona pellucida or implant into uterus

Strain of Origin: mixed

Strain genetic background: 129X1/SvJ

Strain Development: The original chimera was bred to a C57BL/6J female. CD1 was also introduced before backcrossing to 129/Sv.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Scott Bultman, Ph.D., University of North Carolina at Chapel Hill

Primary Reference:

Bultman S, Gebuhr T, Yee D, La Mantia C, Nicholson J, Gilliam A, Randazzo F, Metzger D, Chambon P, Crabtree G, Magnuson T. A Brg1 null mutation in the mouse reveals functional differences among mammalian SWI/SNF complexes. Mol Cell. 2000 Dec;6(6):1287-95. (Medline PMID: 11163203)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011869-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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