Strain Name:
C3.B6-a/Mmmh
Stock Number:
011915-MU
Citation ID:
RRID:MMRRC_011915-MU

Strain Information

A
Name: nonagouti; wild-type agouti
Synonyms: dark-bellied agouti
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Spontaneous Mutation
a
Name: nonagouti
Synonyms: agouti, As, ASP, agouti signal protein
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Genetic Alterations
This is a spontaneous mutation that disrupts binding of the agouti signaling protein (an inhibitor of melanocortin binding) to the melanocortin receptor. The result is full activity of the melanocortin receptor, resulting in the production of eumelanin (results in a black coat color) by melanocytes.
Genotype Determination
Phenotype
Homozygous: black coat color for C3H/HeJ-a/a

Heterozygous: C3H/HeJ-A/a heterozygotes are agouti but are darker (more black) than the C3H/HeJ-A/A homozygous wild type
Mammalian Phenotype Terms
Allelic Composition: A/A (Genetic Background: Not Specified )

  • normal phenotype
  • pigmentation
Allelic Composition: Ahvy/A (Genetic Background: C3H/HeJ )

Allelic Composition: Aiy/A (Genetic Background: C3H/HeJ-Aiy )

Allelic Composition: Ay/A (Genetic Background: C3HeB/FeJ )

Allelic Composition: Aw-47J/A (Genetic Background: C57BL/6J )

Allelic Composition: A/atl (Genetic Background: involves: 101/H * C3H/HeH )

  • integument
  • pigmentation
Allelic Composition: Ay/A (Genetic Background: involves: C57BL/6 )

Allelic Composition: Ay-Jkn/A (Genetic Background: involves: C57BL/6N )

Allelic Composition: A/atwp (Genetic Background: Not Specified )

MeSH Terms
  • Animals
  • Cell Culture Techniques/methods
  • Chromosome Mapping
  • Cyclin-Dependent Kinase Inhibitor p16/genetics
  • Ethanol/toxicity
  • Genes, ras
  • Karyotyping
  • Melanoma, Experimental/chemically induced
  • Melanoma, Experimental/genetics
  • Melanoma, Experimental/pathology
  • Mice
  • Mice, Inbred C3H
  • Neoplasms, Radiation-Induced/chemically induced
  • Neoplasms, Radiation-Induced/genetics
  • Neoplasms, Radiation-Induced/pathology
  • Tumor Cells, Cultured
  • Ultraviolet Rays
  • Anthraquinones
  • Carcinogens/toxicity
  • Emodin/toxicity
  • Female
  • Melanoma/chemically induced
  • Melanoma/etiology
  • Neoplasms, Radiation-Induced
  • Skin Neoplasms/chemically induced
  • Skin Neoplasms/etiology
Strain Development
C3H/HeJ crossed with C57BL/6 and F1 progeny backcrossed to C3H/HeJ 1 generation by Lynn Lamoreux at Texas A&M University. The F1 mice were sent to donor and were backcrossed for 6 more generations onto C3H/HeJ (using commercially available female C3H/HeJ mice for each new generation). After 7 generations of backcross onto the C3H/HeJ background, donor performed sib-sib matings.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Dermatology
  • Research Tools
Donor
Faith Strickland, Ph.D., Henry Ford Hospital
Primary Reference
  • Strickland FM, Pathak S, Multani AS, Pelley RP, Donawho CK. Molecular characterization of new melanoma cell lines from C3H mice induced by ethanol plus ultraviolet radiation. Cancer Res. 2003 Jul 1;63(13):3503-10. (Medline PMID: 12839934)
  • Strickland FM, Muller HK, Stephens LC, Bucana CD, Donawho CK, Sun Y, Pelley RP. Induction of primary cutaneous melanomas in C3H mice by combined treatment with ultraviolet radiation, ethanol and aloe emodin. Photochem Photobiol. 2000 Sep;72(3):407-14. (Medline PMID: 10989613)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011915-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
011915-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.