Strain Detail Sheet

Strain Name    :

C57BL/6-Tg(APOE-ABCA1)30Ssf/Mmmh

Stock Number :

011941-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(APOE-ABCA1)30Ssf
Name: transgene insertion 30, Silvia Santamarina-Fojo
Alteration at locus: Transgenic
Transgene: ABCA1 (Human)
Name: ATP-binding cassette, sub-family A (ABC1), member 1
Alteration at locus: Transgenic
Promoter: APOE (Human)
Name: apolipoprotein E
Alteration at locus: Transgenic

Genetic Alterations:
The transgene consists of the human apoE promoter, the human ABCA1 cDNA, the poly(A) signal from the human apoE gene, and the hepatic and macrophage control regions of the apoE/apoC-I locus.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Human ABCA1 mRNA and protein are expressed in liver.

Hemizygous phenotype: Similar to Homozygous phenotype.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Tg(APOE-ABCA1)30Ssf/?
        C57BL/6N-Tg(APOE-ABCA1)30Ssf
  • cellular phenotype
    • increased cholesterol efflux (MGI Ref ID J:70494)
      • absolute delivery of HDL-CE to liver is increased 1.5 times
  • homeostasis/metabolism phenotype
    • abnormal apolipoprotein level (MGI Ref ID J:70494)
      • increase in APOA-I, APOA-II and APOB levels in both males and females
      • abnormal circulating apolipoprotein E level (MGI Ref ID J:70494)
        • APOE levels are increased only in males
    • increased cholesterol efflux (MGI Ref ID J:70494)
      • absolute delivery of HDL-CE to liver is increased 1.5 times
    • increased circulating cholesterol level (MGI Ref ID J:70494)
      • fasting plasma total cholesterol, cholesterol esters and free cholesterol levels are elevated as compared to controls
      • increased circulating HDL cholesterol level (MGI Ref ID J:70494)
        • elevated HDL-C
    • increased circulating phospholipid level (MGI Ref ID J:70494)
    • increased liver cholesterol level (MGI Ref ID J:70494)
      • increase in cholesterol and phospholipid content of bile
    • increased triglyceride level (MGI Ref ID J:70494)
      • increased tryiglyceride levels only observed in males
  • liver/biliary system phenotype
    • increased liver cholesterol level (MGI Ref ID J:70494)
      • increase in cholesterol and phospholipid content of bile

Strain of Origin: C57BL/6

Strain genetic background: C57BL/6

Strain Development: The ABCA1 transgene was injected into male pronuclei of C57BL/6 mice. The founder transgenic as bred to C57BL/6 and the line was eventually made homozygous for the transgene in the C57BL/6 background.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease

Strain Origin

Donor: Silvia Santamarina-Fojo, M.D., Ph.D., NIH, NHLBI

Primary Reference:

  • Vaisman BL, Lambert G, Amar M, Joyce C, Ito T, Shamburek RD, Cain WJ, Fruchart-Najib J, Neufeld ED, Remaley AT, Brewer HB Jr, Santamarina-Fojo S. ABCA1 overexpression leads to hyperalphalipoproteinemia and increased biliary cholesterol excretion in transgenic mice. J Clin Invest. 2001 Jul;108(2):303-9. (Medline PMID: 11457883)
  • Joyce CW, Amar MJ, Lambert G, Vaisman BL, Paigen B, Najib-Fruchart J, Hoyt RF Jr, Neufeld ED, Remaley AT, Fredrickson DS, Brewer HB Jr, Santamarina-Fojo S. The ATP binding cassette transporter A1 (ABCA1) modulates the development of aortic atherosclerosis in C57BL/6 and apoE-knockout mice. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):407-12. Epub 2001 Dec 18. (Medline PMID: 11752403)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011941-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
011941-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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