Strain Name:
B6.129S6-Gcktm1.1Mgn/Mmnc
Stock Number:
011949-UNC
Citation ID:
RRID:MMRRC_011949-UNC

Gene Information

Allele Symbol: Gcktm1.1Mgn (Mus musculus (mouse))
Name: glucokinase; targeted mutation 1.1, Mark A Magnuson
Synonyms: gk
Chromosome: 8
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:2177709
Gene Symbol: Gck (Mus musculus (mouse))
Name: glucokinase
Synonyms: Gls006, HK4, MODY2, hexokinase 4, Hlb62
Chromosome: 11
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:1270854
Genetic Alterations
A gene targeting strategy was used to flank exons 9 and 10 in the glucokinase gene with two tandomly-oriented loxP sites.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TL1/TL-1 derived from 129S6/SvEvTac
    Phenotype
    Homozygous: Homozygous mice are viable. Blood glucose concentrations in the gk-lox/lox mice are slightly higher than wild types (194 3 mg/dl vs. 175 8 mg/dl) however the mice are otherwise normal. This finding suggests that the insertion of a loxP site (and some flanking sequences) between exons 8 and 9 may have caused a slight attenuation in glucokinase gene expression compared to mice with two wild type alleles.

    Heterozygous:
    This strain allows for the tissue specific knock-out of glucokinase, thus generating phenotypically distinguishable mouse strains. For example, crossing the gk-lox/lox mice with an insulin-cre transgenic allows for the cell specific knock-out of glucokinase. The same is true for hepatocytes when gk-lox/lox mice are crossed with albumin-cre transgenics.
    Mammalian Phenotype Terms
    Allelic Composition: Gcktm1.1Mgn/Gcktm1.1Mgn (Genetic Background: 129S6/SvEvTac-Gcktm1.1Mgn )

    Allelic Composition: Gcktm1.1Mgn/Gcktm1.1Mgn (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

    MeSH Terms
    • Albumins/genetics
    • Alleles
    • Animals
    • Base Sequence
    • DNA Primers
    • Gene Deletion
    • Glucokinase/genetics
    • Glucokinase/metabolism
    • Glucose/metabolism
    • Homeostasis
    • Insulin/genetics
    • Integrases/genetics
    • Islets of Langerhans/enzymology
    • Islets of Langerhans/metabolism
    • Liver/enzymology
    • Liver/metabolism
    • Mice
    • Mice, Knockout
    • Transgenes
    • Viral Proteins
    Strain Development
    After achieving germline transmission mice carrying the gk-lox allele were backcrossed for ten generations. A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6.129S6-Gcktm1.1Mgn/Mmnc 11949 C57BL/6J 129S6/SvEvTac 2580 3 0 0
    Suggested Control Mice
    • Wildtype littermates
    • Diabetes/Obesity
    • Metabolism
    • Models for Human Disease
    • Research Tools
    Donor
    Mark A. Magnuson, M.D., Vanderbilt University
    Primary Reference
    Postic C, Shiota M, Niswender KD, Jetton TL, Chen Y, Moates JM, Shelton KD, Lindner J, Cherrington AD, Magnuson MA. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. J Biol Chem. 1999 Jan 1;274(1):305-15. (Medline PMID: 9867845)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    011949-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / $2,263.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3 : 20-40 embryos / aliquot
    011949-UNC-SPERM Cryo-preserved spermatozoa $548.00 / $910.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity2
    011949-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / $4,696.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.