Strain Name:
SWR-Tg(Tcra3A)1Skd/Mmucd
Stock Number:
011952-UCD
Citation ID:
RRID:MMRRC_011952-UCD
Other Names:
SWR.Nuc3A-Tg (TCRa) smd, SWR-Tg(Tcra3A)1Skd/Mmcd

Strain Information

Tcra-V19.1
Name: T cell receptor alpha, variable 19.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Tg(Tcra3A)1Skd
Name: transgene insertion 1, Syamal Datta
Synonyms: 3A TCR alpha-chain, Tg(Tcra-V19.1)1Skd
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Rearranged TCR V alpha 19.1-J alpha 41 transgene was derived from a T helper cell clone (3A) specific for nucleosomes and its immunodominant histone peptide H4 (71- 94). The T cell originated in a lupus-prone mouse called (SWR x NZB)F1 or SNF1, and it induces pathogenic anti-DNA autoantibodies and accelerates lupus nephritis.
Phenotype
Homozygous: Marked deletion of thymocytes (60 to 80%). Increased frequency of T cells responsive to nucleosomes and its histone peptide H4 (71-94) in the periphery.

Heterozygous:
Same as homozygous
MeSH Terms
  • Animals
  • Apoptosis
  • Autoimmunity
  • Dendritic Cells/physiology
  • Histones/pharmacology
  • Lupus Vulgaris/immunology
  • Mice
  • Mice, Inbred NZB
  • Mice, Transgenic
  • Nucleosomes/immunology
  • Receptors, Antigen, T-Cell/physiology
  • T-Lymphocytes/immunology
  • T-Lymphocytes, Regulatory/physiology
  • Thymus Gland/immunology
  • Amino Acid Sequence
  • Antibodies, Monoclonal/immunology
  • Antibodies, Monoclonal/metabolism
  • Autoantibodies/immunology
  • Autoantigens/immunology
  • Autoimmunity/immunology
  • CD4 Antigens/immunology
  • Cloning, Molecular
  • Epitopes/immunology
  • Histocompatibility Antigens Class II/immunology
  • Histones/chemistry
  • Humans
  • Interleukin-2/metabolism
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Peptide Fragments/immunology
  • Receptors, Antigen, T-Cell/chemistry
  • Receptors, Antigen, T-Cell/metabolism
  • Transfection/genetics
  • Adolescent
  • Adult
  • Antibodies, Antinuclear/biosynthesis
  • Autoantigens/metabolism
  • CD4-Positive T-Lymphocytes/immunology
  • CD4-Positive T-Lymphocytes/metabolism
  • Cell Line
  • Clone Cells
  • Coculture Techniques
  • DNA/immunology
  • Epitopes, T-Lymphocyte/immunology
  • Epitopes, T-Lymphocyte/metabolism
  • Female
  • Histones/immunology
  • Leukocytes, Mononuclear/immunology
  • Lupus Erythematosus, Systemic/immunology
  • Lymphocyte Activation
  • Male
  • Middle Aged
  • Peptides/immunology
  • Peptides/metabolism
Strain Development
In 1998, the T cell receptor alpha chain transgene construct was injected directly into the fertilized eggs of SWR mice, which is an inbred strain obtained from Jackson Laboratory, ME. The founder SWR lines were screened by PCR of DNA from tail clippings and interbred to homozygosity for the transgene and has been kept in the inbred SWR background for at least 7 years.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Syamal K. Datta, MBBS, Ph.D., Northwestern University Feinberg Medical School
Primary Reference
  • Michaels MA, Kang HK, Kaliyaperumal A, Satyaraj E, Shi Y, Datta SK. A defect in deletion of nucleosome-specific autoimmune T cells in lupus-prone thymus: role of thymic dendritic cells. J Immunol. 2005 Nov 1;175(9):5857-65. (Medline PMID: 16237078)
  • Shi Y, Kaliyaperumal A, Lu L, Southwood S, Sette A, Michaels MA, Datta SK. Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain. J Exp Med. 1998 Feb 2;187(3):367-78. (Medline PMID: 9449717)
  • Lu L, Kaliyaperumal A, Boumpas DT, Datta SK. Major peptide autoepitopes for nucleosome-specific T cells of human lupus. J Clin Invest. 1999 Aug;104(3):345-55. (Medline PMID: 10430616)

Colony and Husbandry Information

Donor kept mice in barrier spf housing, but the mice are not immunodeficient.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011952-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
011952-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
011952-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.