Strain Name:
STOCK Chek1tm1Sany/Mmucd
Stock Number:
012032-UCD
Citation ID:
RRID:MMRRC_012032-UCD
Other Names:
Chk1SNP156, Chek1SNP156, STOCK Chek1tm1Sany/Mmcd
Major Collection:

Strain Information

Chek1tm1Sany
Name: checkpoint kinase 1; targeted mutation 1, Yolanda Sanchez
Synonyms: Check1SNP156
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Chek1
Name: checkpoint kinase 1
Synonyms: Chk1
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knockout
NCBI: 12649
HGNC: HGNC:1925
Homologene: 975
Genetic Alterations
Chek1 genomic clones were isolated from the RPCI-22 (129S6/SvEvTac) mouse BAC library (ResGen). (ResGen). An Xba I-Hind III fragment containing exons 2-6 was cloned from BAC into the bluescript vector. Mutations were made in exon 5 to change the triplet encoding R156 to that of glutamine (the corresponding SNP in hChek1). Silent mutations were also made nearby to create an Nhe I site and a Kpn I site (not shown) to facilitate the detection of targeted alleles. A neo cassette flanked by FRT sites was then inserted between exons 4 and 5. The resulting construct was digested with restriction enzymes to separate the modified Chek1 fragment from the vector bone; the former was electroporated into mouse embryonic stem cells.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: Undetermined.

Heterozygous: The heterozygous has no phenotype in the absence of challenge and have not determined in the presence of challenge
MeSH Terms
  • 14-3-3 Proteins
  • Amino Acid Sequence
  • Animals
  • CDC2 Protein Kinase/metabolism
  • Cell Cycle Proteins/antagonists & inhibitors
  • Cell Cycle Proteins/metabolism
  • Checkpoint Kinase 1
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11
  • Cytoskeletal Proteins
  • DNA Damage
  • F-Box Proteins
  • F-Box-WD Repeat-Containing Protein 7
  • G2 Phase
  • HeLa Cells
  • Humans
  • Mice
  • Mitosis
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases/metabolism
  • Phosphorylation
  • Phosphoserine/metabolism
  • Protein Kinases/chemistry
  • Protein Kinases/genetics
  • Protein Kinases/metabolism
  • Protein Tyrosine Phosphatases/metabolism
  • Proteins/metabolism
  • Recombinant Fusion Proteins/metabolism
  • Schizosaccharomyces pombe Proteins
  • Signal Transduction
  • Transfection
  • Tyrosine 3-Monooxygenase
  • Ubiquitin-Protein Ligases
  • cdc25 Phosphatases
  • Apoptosis
  • DNA Replication
  • Epithelial Cells/metabolism
  • Epithelial Cells/pathology
  • Genes, Tumor Suppressor
  • Integrases
  • Mammary Glands, Animal/metabolism
  • Mammary Glands, Animal/pathology
  • Mice, Knockout
  • Microfilament Proteins/metabolism
  • Muscle Proteins
  • Phenotype
  • Protein Kinases/physiology
  • S Phase
  • Viral Proteins
  • cdc25 Phosphatases/metabolism
  • Alleles
  • Antineoplastic Agents/pharmacology
  • Ataxia Telangiectasia Mutated Proteins
  • Cell Cycle Proteins
  • Cells, Cultured
  • DNA Repair
  • Female
  • Flow Cytometry
  • Gamma Rays
  • Heterozygote
  • Hydroxyurea/pharmacology
  • In Situ Nick-End Labeling
  • Mammary Neoplasms, Experimental/genetics
  • Mice, Transgenic
  • Mitosis/drug effects
  • Mitosis/radiation effects
  • Models, Genetic
  • Mutagenesis
  • Neoplasm Transplantation
  • Nocodazole/pharmacology
  • Protein-Serine-Threonine Kinases/genetics
  • Protein-Serine-Threonine Kinases/metabolism
  • Stem Cells
  • Time Factors
  • Tumor Suppressor Protein p53/metabolism
  • Ultraviolet Rays
Strain Development
Since the ES cell was 129S6 and the chimeras were crossed with BlackSwiss females, the offspring carrying the mutant Chek1 allele were 50% 129S6 and 50% BlackSwiss background. After the first backcross to C57BL/6 the genetic mix is 1/4 129S6, 1/4 BlackSwiss, 1/2 C57BL/6. After second backcross the mice will be 1/8 129S6, 1/8 BlackSwiss, 3/4 C57BL/6. It is this generation of animals that was deposited to MMRRC. Donor notes no unusual breeding or reproductive characteristics observed.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Yolanda Sanchez, Ph.D., Datrmouth Medical School
Primary Reference
  • Sanchez Y; Wong C; Thoma RS; Richman R; Wu Z; Piwnica-Worms H ; Elledge SJ, Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25 , Science 1997 Sep 5;277(5331):1497-501 (Medline PMID: 9278511)
  • Lam MH; Liu Q; Elledge SJ; Rosen JM, Chk1 is haploinsufficient for multiple functions critical to tumor suppression., Cancer Cell 2004 Jul;6(1):45-59 (Medline PMID: 15261141)
  • Liu Q; Guntuku S; Cui XS; Matsuoka S; Cortez D; Tamai K; Luo G; Carattini-Rivera S; DeMayo F; Bradley A; Donehower LA; Elledge SJ, Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint., Genes Dev 2000 Jun 15;14(12):1448-59 (Medline PMID: 10859164)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
012032-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
012032-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
012032-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.