Allele Symbol: Chek1tm1Sany
Name: targeted mutation 1, Yolanda Sanchez
Alteration at locus: Knockout
Gene Symbol: Chek1
Name: checkpoint kinase 1
Alteration at locus: Knockout
ES Cell Line: Not specified derived from 129S6 x BlackSwiss
Heterozygous phenotype: The heterozygous has no phenotype in the absence of challenge and have not determined in the presence of challenge
Strain of Origin: 50% 129S6 and 50% BlackSwiss background
Strain genetic background: C57BL/6
Strain Development: Since the ES cell was 129S6 and the chimeras were crossed with BlackSwiss females, the offspring carrying the mutant Chek1 allele were 50% 129S6 and 50% BlackSwiss background. After the first backcross to C57BL/6 the genetic mix is 1/4 129S6, 1/4 BlackSwiss, 1/2 C57BL/6. After second backcross the mice will be 1/8 129S6, 1/8 BlackSwiss, 3/4 C57BL/6. It is this generation of animals that was deposited to MMRRC. Donor notes no unusual breeding or reproductive characteristics observed.
Suggested Control Mice:
Donor: Yolanda Sanchez, Ph.D., Datrmouth Medical School
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.