Strain Detail Sheet

Strain Name    :

STOCK Chek1tm1Sany/Mmucd

Stock Number :

012032-UCD

Other Names   :

Chk1SNP156, Chek1SNP156, STOCK Chek1tm1Sany/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Chek1tm1Sany
Name: targeted mutation 1, Yolanda Sanchez
Alteration at locus: Knockout
Gene Symbol: Chek1
Name: checkpoint kinase 1
Chromosome: 9
Alteration at locus: Knockout

Genetic Alterations:
Chek1 genomic clones were isolated from the RPCI-22 (129S6/SvEvTac) mouse BAC library (ResGen). (ResGen). An Xba I-Hind III fragment containing exons 2-6 was cloned from BAC into the bluescript vector. Mutations were made in exon 5 to change the triplet encoding R156 to that of glutamine (the corresponding SNP in hChek1). Silent mutations were also made nearby to create an Nhe I site and a Kpn I site (not shown) to facilitate the detection of targeted alleles. A neo cassette flanked by FRT sites was then inserted between exons 4 and 5. The resulting construct was digested with restriction enzymes to separate the modified Chek1 fragment from the vector bone; the former was electroporated into mouse embryonic stem cells.

Genotype Determination:

ES Cell Line: Not specified derived from 129S6 x BlackSwiss

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype:

Heterozygous phenotype: The heterozygous has no phenotype in the absence of challenge and have not determined in the presence of challenge

Strain of Origin: 50% 129S6 and 50% BlackSwiss background

Strain genetic background: C57BL/6

Strain Development: Since the ES cell was 129S6 and the chimeras were crossed with BlackSwiss females, the offspring carrying the mutant Chek1 allele were 50% 129S6 and 50% BlackSwiss background. After the first backcross to C57BL/6 the genetic mix is 1/4 129S6, 1/4 BlackSwiss, 1/2 C57BL/6. After second backcross the mice will be 1/8 129S6, 1/8 BlackSwiss, 3/4 C57BL/6. It is this generation of animals that was deposited to MMRRC. Donor notes no unusual breeding or reproductive characteristics observed.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Yolanda Sanchez, Ph.D., Datrmouth Medical School

Primary Reference:

  • Sanchez Y; Wong C; Thoma RS; Richman R; Wu Z; Piwnica-Worms H ; Elledge SJ, Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25 , Science 1997 Sep 5;277(5331):1497-501 (Medline PMID: 9278511)
  • Lam MH; Liu Q; Elledge SJ; Rosen JM, Chk1 is haploinsufficient for multiple functions critical to tumor suppression., Cancer Cell 2004 Jul;6(1):45-59 (Medline PMID: 15261141)
  • Liu Q; Guntuku S; Cui XS; Matsuoka S; Cortez D; Tamai K; Luo G; Carattini-Rivera S; DeMayo F; Bradley A; Donehower LA; Elledge SJ, Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint., Genes Dev 2000 Jun 15;14(12):1448-59 (Medline PMID: 10859164)

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
012032-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
012032-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
012032-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/23/2014