Strain Detail Sheet

Strain Name    :

C.A-Thy1a/Mmnc

Stock Number :

000123-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Thy1a
Name: a variant
Chromosome: 9
Alteration at locus: Polymorphic allele
Gene Symbol: Thy1
Name: thymus cell antigen 1, theta
Chromosome: 9
Alteration at locus: Polymorphic allele

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Target gene is a cell surface marker found on T cells and limited other tissues. This allele is commonly used to differentiate T cell subsets in mixed lymphocyte or bone marrow tranplantation experiments. This line is particularly valuable when combined with TCR-transgenic strains restricted to the BALB/c (H-2d) background. One such strain is the widely used DO11.10 which expresses a transgenic TCR specific for a peptide sequence of ovalbumin. Phenotype can be detected by immunostaining of T cells with specific commercially-available monoclonal antibodies.

Strain of Origin: A

Strain Development: The strain was established by Dr. Shimon Sakaguchi (Japan) by backcrossing A-Thy-1.1 congenic mice, originally made by Edward Boyse at Sloan Kettering Institute, to BALB/c mice 11 times and brother-sister matings thereafter. Brother-sister matings are more than 30 generations. Mice are homozygous for the Thy 1.1 allele.

Suggested Control Mice:

  • BALB/c

Research Applications

  • Transplantation
  • Immunology
  • T cell
  • Thy1.1 allele

Strain Origin

Donor: Dr. V. Godfrey, University of North Carolina, Chapel Hill
Dr. Shimon Sakaguchi, Kyoto University, Japan

Primary Reference:

  • Sakaguchi S, Sakaguchi N, Shimizu J, Yamazaki S, Sakihama T, Itoh M, Kuniyasu Y, Nomura T, Toda M, Takahashi T. Immunologic tolerance maintained by CD25+ CD4+ regulatory T cells: their common role in controlling autoimmunity, tumor immunity, and transplantation tolerance. Immunol Rev. 2001 Aug;182:18-32. Review. (Medline PMID: 11722621)
  • Sakaguchi S. Animal models of autoimmunity and their relevance to human diseases. Curr Opin Immunol. 2000 Dec;12(6):684-90. (Medline PMID: 11102773)
  • Sakaguchi S, Sakaguchi N. Organ-specific autoimmune disease induced in mice by elimination of T cell subsets. V. Neonatal administration of cyclosporin A causes autoimmune disease. J Immunol. 1989 Jan 15;142(2):471-80. (Medline PMID: 2783437)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Appearance

Coat color: White

Other:

Breeding

MMRRC Breeding System: Intra-strain mating

Breeding Scheme(s):

  • Homozygous female x Homozygous male

Generation: N11 (prior to subsequent inbreeding); F30+

Overall Breeding Performance: Excellent

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 28 weeks 28 weeks

Average litter size: 6

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000123-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/21/2014