Strain Name:
129S-Pitx2tm1Jfm/Mmucd
Stock Number:
000124-UCD
Citation ID:
RRID:MMRRC_000124-UCD
Other Names:
pitx2 delta abc null, 129S-Pitx2tm1Jfm/Mmcd

Strain Information

Pitx2tm1Jfm
Name: paired-like homeodomain transcription factor 2; targeted mutation 1, James F Martin
Synonyms: delta abcnull, pitx2hd
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Targeted Mutation
Pitx2
Name: paired-like homeodomain transcription factor 2
Synonyms: Ptx2, Pitx2c, Brx1a, solurshin, Munc30, Rieg, Pitx2a, Brx1, Otlx2, Brx1b, Pitx2b
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Targeted Mutation
NCBI: 18741
HGNC: HGNC:9005
Homologene: 55454
Genetic Alterations
A neomycin resistance cassette replaced exons 5 and part of exon 6, which encodes the homeobox domain.
Genotype Determination
ES Cell Line
AK7
Phenotype
Homozygous embryonic lethal - defects in left right asymmetry and craniofacial development and ocular development. Heterozygous mice have anterior chamber defects of the eye. 129 background males tend to develop rectal prolapse.
Mammalian Phenotype Terms
Allelic Composition: Pitx2tm1Jfm/Pitx2tm1Jfm (Genetic Background: involves: 129S4/SvJaeSor )

Allelic Composition: Pitx2tm1Jfm/Pitx2+ (Genetic Background: involves: 129S4/SvJaeSor )

Allelic Composition: Pitx2tm1Jfm/Pitx2tm4(cre)Jfm (Genetic Background: involves: 129S4/SvJaeSor )

Allelic Composition: Pitx2tm1Jfm/Pitx2tm2Jfm (Genetic Background: involves: 129S4/SvJaeSor )

Allelic Composition: Pitx2tm1Jfm/Pitx2tm2Jfm (Genetic Background: involves: 129S4/SvJaeSor * C57BL/6J )

Allelic Composition: Pitx2tm1Jfm/Pitx2tm2.1Jfm (Genetic Background: involves: 129S4/SvJaeSor * C57BL/6J )

MeSH Terms
  • Abnormalities, Multiple/embryology
  • Abnormalities, Multiple/genetics
  • Alleles
  • Animals
  • Body Patterning/genetics
  • Craniofacial Abnormalities/embryology
  • Craniofacial Abnormalities/genetics
  • Gene Targeting
  • Heart/embryology
  • Heart Defects, Congenital/embryology
  • Heart Defects, Congenital/genetics
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology
  • Lung/abnormalities
  • Lung/embryology
  • Mice
  • Mutation
  • Nuclear Proteins
  • Paired Box Transcription Factors
  • Syndrome
  • Transcription Factors/genetics
  • Transcription Factors/physiology
Strain Development
A neo cassette targeting Pitx2 was electroporated into AK7 ES cells, creating a deletion of exon 5 and most of exon 6. Cells were microinjected to blastocysts, and chimera were mated to 129S6/SvEvTac for generation of heterozygous progeny. The colony was maintained using heterozygous males and 129S6 females.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Developmental Biology
  • Models for Human Disease
Donor
James Martin, M.D., Ph.D., Texas A&M Health Science Center
Primary Reference
Lu MF, Pressman C, Dyer R, Johnson RL, Martin JF. Function of Rieger syndrome gene in left-right asymmetry and craniofacial development. Nature. 1999 Sep 16;401(6750):276-8. (Medline PMID: 10499585)

Colony and Husbandry Information

Homozygous are 14.5 dpc embroyonic lethal

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Heterozygous female x Wildtype male
  • Wildtype female x Heterozygous male
Generation
Backcross unknown, created on 129S4 (AK7) ES cells backcrossed to 129S females, number of times not given. F3+ at MMRRC UCD
Overall Breeding Performance
Fair
Average litter size
4 to 6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000124-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000124-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
000124-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.