Strain Name:
B6.129P2-Sphk2Gt(pGT1dTMpfs)1Kly/Mmnc
Stock Number:
012831-UNC
Citation ID:
RRID:MMRRC_012831-UNC
Other Names:
sphingosine kinase 2

Strain Information

Sphk2Gt(NPX039)Byg
Name: sphingosine kinase 2; gene trap NPX039, BayGenomics
Synonyms: Sphk2Gt(pGT1dTMpfs)1Kly
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Gene Trap
Sphk2
Name: sphingosine kinase 2
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Gene Trap
NCBI: 56632
Homologene: 32456
Genetic Alterations
Gene trap inserted into the intron immediately before the first protein coding exon.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    E14TG2a.4 derived from 129P2/OlaHsd
    Phenotype
    Homozygous: Reduced fertility (females), lower circulating sphingosine 1-phosphate levels, inability to activate S1P prodrugs such as FTY720 (fingolimod), no detectable Sphk2 activity.

    Heterozygous: Not characterized
    Mammalian Phenotype Terms
    Allelic Composition: Sphk2Gt(NPX039)Byg/Sphk2Gt(NPX039)Byg (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    MeSH Terms
    • Animals
    • Fingolimod Hydrochloride
    • Flow Cytometry
    • Homozygote
    • Immunosuppressive Agents/metabolism
    • Immunosuppressive Agents/toxicity
    • Lymphocytes/drug effects
    • Lymphocytes/enzymology
    • Lymphopenia/chemically induced
    • Lymphopenia/enzymology
    • Lysophospholipids/metabolism
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Phosphorylation/drug effects
    • Phosphotransferases (Alcohol Group Acceptor)/genetics
    • Phosphotransferases (Alcohol Group Acceptor)/physiology
    • Prodrugs/metabolism
    • Prodrugs/toxicity
    • Propylene Glycols/metabolism
    • Propylene Glycols/toxicity
    • Reverse Transcriptase Polymerase Chain Reaction
    • Sphingosine/analogs & derivatives
    • Sphingosine/metabolism
    Strain Development
    Obtained trapped ES cell line NPX-039 from MMRRC, made mouse, crossed to obtain tr/tr mice, used speed congenics to make congenic on C57BL/6 background.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Diabetes/Obesity
    • Immunology and Inflammation
    Donor
    Kevin R. Lynch, Ph.D., University of Virginia
    Primary Reference
    Kharel Y, Lee S, Snyder AH, Sheasley-O'neill SL, Morris MA, Setiady Y, Zhu R, Zigler MA, Burcin TL, Ley K, Tung KS, Engelhard VH, Macdonald TL, Pearson-White S, Lynch KR. Sphingosine kinase 2 is required for modulation of lymphocyte traffic by FTY720. J Biol Chem. 2005 Nov 4;280(44):36865-72. Epub 2005 Aug 10. (Medline PMID: 16093248)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    012831-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.