Strain Name:
B6.Cg-Tg(CAG-WRN*K577M)5025Wcl/Mmmh
Stock Number:
000130-MU
Citation ID:
RRID:MMRRC_000130-MU
Major Collection:

Strain Information

WRN
Name: Werner synd
Type: Gene
Species: Homo sapiens (human)
Chromosome: 8
Tg(CAG-WRN*K577M)5025Wcl
Name: transgene insertion 5025, Warren C Ladiges
Synonyms: Tg(CAG-WRN*K577M)5025Wcl, K577-WRN, Tg(ACTB-WRN*K577M)5025Wcl
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Single amino acid substitution at position 577 (K577M) in the recombinant human WRNp gene.
Genotype Determination
Phenotype
1. Hypersensitivity to 4-nitroquinoline-1-oxide
2. Reduced replicative potential
3. Reduced expression of the endogenous WRN protein
MeSH Terms
  • 4-Nitroquinoline-1-oxide/analogs & derivatives
  • 4-Nitroquinoline-1-oxide/pharmacology
  • Alleles
  • Animals
  • Cell Division
  • DNA Helicases/genetics
  • Down-Regulation
  • Exodeoxyribonucleases
  • Genes, Dominant
  • Humans
  • Mice
  • Mice, Transgenic
  • Phenotype
  • Quinolones/pharmacology
  • RecQ Helicases
  • Werner Syndrome/genetics
  • Werner Syndrome Helicase
Suggested Control Mice
  • Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Models for Human Disease
  • Biology of Aging
Donor
Warren C. Ladiges, D.V.M., University of Washington.
Primary Reference
Wang L, Ogburn CE, Ware CB, Ladiges WC, Youssoufian H, Martin GM, Oshima J. Cellular Werner phenotypes in mice expressing a putative dominant-negative human WRN gene. Genetics. 2000 Jan;154(1):357-62. (Medline PMID: 10628995)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • C57BL/6 female x Heterozygous male
Generation
Unknown
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Unknown Unknown
Age Reproductive Decline: Unknown Unknown
Average litter size
8 - 10
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000130-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000130-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000130-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.