Strain Name:
D1.Cg-Tg(Tcra172,Tcrb172)1Wcl/Mmmh
Stock Number:
000131-MU
Citation ID:
RRID:MMRRC_000131-MU
Other Names:
D1.Cg-Tg(Tcra-V11.1,Trbv13-2)1Wcl/Mmmh
Major Collection:

Strain Information

Tcra-V11.1
Name: T cell receptor alpha, variable 11.1
Synonyms: AV11S1, Va11.1b, Va11.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Trbv13-2
Name: T cell receptor beta, variable 13-2
Synonyms: Tcrb-V8.2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 100124675
Tg(Tcra172,Tcrb172)1Wcl
Name: transgene insertion 1, Warren C Ladiges
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Rearranged Valpha11.1 and Vbeta8.2 TCR chain genes, including the endogenous promoter regions, were isolated from Type II collagen-specific T-cell hybridoma 172 and ligated together to make the transegnic construct.
Genotype Determination
Phenotype
Rapid onset of arthritis with severe clinical signs was detected in transgenic mice after immunization with CII in complete Freund's adjuvant. Histological analysis of inflamed joints resemble arthritic human joints.
MeSH Terms
  • Animals
  • Arthritis, Rheumatoid/immunology
  • Arthritis, Rheumatoid/pathology
  • B-Lymphocytes
  • CD4-Positive T-Lymphocytes/immunology
  • Collagen/immunology
  • Disease Models, Animal
  • Flow Cytometry
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Hybridomas
  • Immune Tolerance
  • Joints/pathology
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred DBA
  • Mice, Transgenic
  • Receptors, Antigen, T-Cell, alpha-beta/genetics
  • Receptors, Antigen, T-Cell, alpha-beta/immunology
  • Spleen/cytology
  • Spleen/immunology
  • Thymus Gland/cytology
  • Thymus Gland/immunology
  • Transgenes
  • Adoptive Transfer
  • Arthritis, Rheumatoid/therapy
  • Genetic Therapy/methods
  • Interleukin-12/antagonists & inhibitors
  • Male
  • Retroviridae/genetics
  • T-Lymphocyte Subsets/immunology
  • T-Lymphocyte Subsets/transplantation
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Warren C. Ladiges, D.V.M., University of Washington.
Primary Reference
  • Osman GE, Cheunsuk S, Allen SE, Chi E, Liggitt HD, Hood LE, Ladiges WC. Expression of a type II collagen-specific TCR transgene accelerates the onset of arthritis in mice. Int Immunol. 1998 Nov;10(11):1613-22. (Medline PMID: 9846690)
  • Nakajima A, Seroogy CM, Sandora MR, Tarner IH, Costa GL, Taylor-Edwards C, Bachmann MH, Contag CH, Fathman CG. Antigen-specific T cell-mediated gene therapy in collagen-induced arthritis. J Clin Invest. 2001 May;107(10):1293-301. (Medline PMID: 11375419)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000131-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000131-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.