Strain Name    : D2.Cg-Ay/Mmmh

Stock Number : 000137-MU

Gene Information

(provided by MGI)
Allele Symbol: A
Name: wild-type agouti
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Allele Symbol: Ay
Name: agouti yellow
Chromosome: 2
Alteration at locus: Spontaneous Mutation
Genetic Alterations:
The spontaneous mutation appears to be a DNA structural alteration that disrupts a gene, hnRNP-associated with lethal yellow (Raly), 5' to the agouti locus and places the agouti locus under the control of the Raly promotor.

Genotype Determination:

ES Cell Line: R1

Phenotype

The agouti yellow gene is homozygous lethal. Therefore nonagouti is also segregating. As is true of all DBA mice, these are also mutant at the dilute (Myo5a) and brown (Tyrp1) loci. For this reason, the homozygous nonagouti littermates are dilute brown in phenotype and the heterozygous mice are dilute yellow in phenotype. The full genotype of yellow mice is DBA/2J-Ay/a, Myo5ad/Myo5ad, Tyrp1b/Tyrp1b; the control littermates are DBA/2J-a/a, Myo5ad/Myo5ad, Tyrp1b/Tyrp1b.

Mice heterozygous for the mutant allele have a dilute yellow coat, are obese, but not as obese as C57BL/6J-Ay mice. Yellow is dominant to nonagouti and epistatic to brown. It has not been determined if the yellow mice are prone to diabetes on the DBA background, as are the C57BL/6J-Ay mice.

Mammalian Phenotype Terms:
Allelic Composition: A/A (Genetic Background: Not Specified )

  • normal phenotype
  • pigmentation
Allelic Composition: Ahvy/A (Genetic Background: C3H/HeJ )

Allelic Composition: Aiy/A (Genetic Background: C3H/HeJ-Aiy )

Allelic Composition: Ay/A (Genetic Background: C3HeB/FeJ )

Allelic Composition: Aw-47J/A (Genetic Background: C57BL/6J )

Allelic Composition: A/atl (Genetic Background: involves: 101/H * C3H/HeH )

  • integument
  • pigmentation
Allelic Composition: Ay/A (Genetic Background: involves: C57BL/6 )

Allelic Composition: Ay-Jkn/A (Genetic Background: involves: C57BL/6N )

Allelic Composition: A/atwp (Genetic Background: Not Specified )

Allelic Composition: Ay/Ay (Genetic Background: 101-Ay )

  • mortality/aging
  • other phenotype
Allelic Composition: Ay/Aw (Genetic Background: 129S1.C3-Ay/Aw Chr 19MOLF/Ei )

Allelic Composition: Ay/a (Genetic Background: B6.Cg-Ay/J )

Allelic Composition: Ay/A (Genetic Background: C3HeB/FeJ )

Allelic Composition: Ay/A (Genetic Background: involves: C57BL/6 )

Allelic Composition: Ay/a (Genetic Background: involves: KK )

Strain of Origin: old mutant of the mouse fancy

Strain genetic background: DBA/2J

Strain Development: This strain was created by backcrossing the spontaneous mutation from C57BL/6J-Ay/a, that was obtained from JAX, onto DBA/2J.

Suggested Control Mice:

  • Wildtype littermates
  • DBA/2J

  • Pigmentation
  • Obesity
  • Reproduction

Donor: Dr. M. L. Lamoreux, Texas A&M University

Primary Reference:

  • Michaud EJ, Bultman SJ, Klebig ML, van Vugt MJ, Stubbs LJ, Russell LB, Woychik RP. A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2562-6. (Medline: 8146154)
  • Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline: 11277491)
  • Ollmann MM, Lamoreux ML, Wilson BD, Barsh GS. Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo. Genes Dev. 1998 Feb 1;12(3):316-30. (Medline: 9450927)

Colony and Husbandry Information

Do not feed high fat diet due to obesity and diabetic tendencies. Pups are small and should not be weaned until at least 4-5 weeks of age (the donating investigator routinely waits to wean these mice until they are 8-10 weeks of age). Males seem to mature slowly and don't breed well until they are 3 months of age.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Dilute yellow

Other:


MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • Wildtype Female x Heterozygous Male
  • DBA/2J Female x Heterozygous Male
  • Heterozygous females are fertile, but resulting pups have a poor survival rate to weaning age. Recommend mating heterozygous males to DBA/2J or wildtype females.

Generation: N10+

Overall Breeding Performance: Poor. It is usually very much easier to obtain viable litters if yellow males are mated with nonyellow females, and if the mice are maintained in more than usually crowded conditions. Trying to mate these as pairs probably would not work. Some of the yellow females never breed. So in rederivation, it will be necessary to provide more than three animals per client.

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 6 months 6 months

Average litter size: 6

Recommended wean age: 5 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
000137-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000137-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.