Strain Name:
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Strain Information

Name: RPTOR independent companion of MTOR, complex 2; targeted mutation 1.1, Mark A Magnuson
Synonyms: ricEx3cond
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Targeted Mutation
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Targeted Mutation
NCBI: 78757
VEGA: 15
Homologene: 34317
Genetic Alterations
This conditional allele resulted from a multi-allelic gene targeting strategy that involved the use of both FRT and loxP sites. This pia[lox] allele contains a single (remnant) FRT site and two loxP sites flanking exon 3 of this gene.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TL1/TL-1 derived from 129S6/SvEvTac
    Homozygous: Homozygous animals are viable and do not exhibit any obvious mutant phenotype.

    Heterozygous: Heterozygous animals are viable and do not exhibit any obvious mutant phenotype.
    MeSH Terms
    • Actins/metabolism
    • Alleles
    • Animals
    • Carrier Proteins/metabolism
    • Cell Proliferation
    • Cells, Cultured
    • Embryo, Mammalian/cytology
    • Fetal Development/genetics
    • Fetal Viability/genetics
    • Fibroblasts/metabolism
    • Fibroblasts/physiology
    • Gene Targeting
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Molecular Sequence Data
    • Protein Kinases/analysis
    • Protein Kinases/metabolism
    • Proto-Oncogene Proteins c-akt/metabolism
    • Rapamycin-Insensitive Companion of mTOR Protein
    • TOR Serine-Threonine Kinases
    Strain Development

    After achieving germline transmission of a pia[lacZ+neo] allele that was generated by gene targeting in ES cells, mice were crossed with Flpe transgenic mice. This resulted in the creation of the pia[lox] allele. Mice carrying the pia[lox] allele, which were in a mixed genetic background, have been backcrossed to C57BL/6J.

    A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6.129S6(SJL)-Rictortm1.1Mgn/Mmnc 14113 C57BL/6J 129S6 2516 0 66 0
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact Older strains may not have this information.
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Developmental Biology
    • Diabetes/Obesity
    • Hematology
    • Immunology and Inflammation
    • Internal/Organ
    • Metabolism
    • Models for Human Disease
    • Neurobiology
    • Reproduction
    • Research Tools
    Mark A. Magnuson, M.D., Vanderbilt University
    Primary Reference
    Shiota C, Woo JT, Lindner J, Shelton KD, Magnuson MA. Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability. Dev Cell. 2006 Oct;11(4):583-9. Epub 2006 Sep 7. (Medline PMID: 16962829)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    014113-UNC-SPERM Cryo-preserved spermatozoa $564.00 / $937.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    014113-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to for more information.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.