After achieving germline transmission of a pia[lacZ+neo] allele that was generated by gene targeting in ES cells, mice were crossed with Flpe transgenic mice. This resulted in the creation of the pia[lox] allele. Mice carrying the pia[lox] allele, which were in a mixed genetic background, have been backcrossed to C57BL/6J.
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
|MMRRC Strain||Reference Strains||Genomic makeup (Mb)|
|Strain Name||MMRRC ID #||Reference 1||Reference 2||Ref 1||Ref 2||Het||Unknown|
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
|MMRRC Item #||Description||Distribution Fee / Unit (US $)
*Shipping & Handling not included*
|014113-UNC-SPERM||Cryo-preserved spermatozoa||$564.00 / $937.00
Non-Profit / For-Profit
|014113-UNC-RESUS||Litter recovered from cryo-archive||$2,914.00 / $4,837.00
Non-Profit / For-Profit
|Litter||Recovered litter4; additional fees for any special requests.|
|Cryopreserved material may be available upon request, please inquire to firstname.lastname@example.org for more information.|
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.