Strain Name:
STOCK Pik3r1tm2Dfr/Mmucd
Stock Number:
014244-UCD
Citation ID:
RRID:MMRRC_014244-UCD
Other Names:
Pik3r1-null

Strain Information

Pik3r1tm1Dfr
Name: phosphoinositide-3-kinase regulatory subunit 1; targeted mutation 1, David A Fruman
Synonyms: p85a-p55a-p50a-, p85alpha-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Targeted Mutation
Pik3r1
Name: phosphoinositide-3-kinase regulatory subunit 1
Synonyms: p85alpha, p50alpha, p55alpha, PI3K
Type: Gene
Species: Mouse
Chromosome: 13
Alteration at locus: Targeted Mutation
NCBI: 18708
HGNC: HGNC:8979
Homologene: 7889
Genetic Alterations
A neocassette replaces exon 7 and some intronic DNA.
ES Cell Line
TC1/TC-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Perinatal lethal due to liver necrosis. Other phenotypes include chylous ascites, hypoglycemica, enlarged muscle fibers, impaired erythropoiesis and impaired B cell development. Mice are severely hypoglycemic.

Heterozygous: Hypoglycemia, increased insulin sensitivy and glucose tolerance. This has been shown in the mixed background but not tested in BalB/C background.
Mammalian Phenotype Terms
Allelic Composition: Pik3r1tm1Dfr/Pik3r1tm1Dfr (Genetic Background: involves: 129S6/SvEvTac )

Allelic Composition: Pik3r1tm1Dfr/Pik3r1tm1Dfr (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

MeSH Terms
  • Animals
  • Apoptosis
  • B-Lymphocytes/cytology
  • B-Lymphocytes/enzymology
  • B-Lymphocytes/immunology
  • Catalytic Domain
  • Cell Cycle
  • Chimera
  • Chromones/pharmacology
  • Enzyme Inhibitors/pharmacology
  • Female
  • Gene Targeting
  • Immunoglobulins/blood
  • Leukocyte Common Antigens/analysis
  • Lymphocyte Activation
  • Lymphocyte Count
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Morpholines/pharmacology
  • Phosphatidylinositol 3-Kinases/antagonists & inhibitors
  • Phosphatidylinositol 3-Kinases/genetics
  • Phosphatidylinositol 3-Kinases/metabolism
  • Protein-Tyrosine Kinases/genetics
  • Protein-Tyrosine Kinases/metabolism
  • Spleen/immunology
  • T-Lymphocytes/cytology
  • T-Lymphocytes/enzymology
  • T-Lymphocytes/immunology
  • Abnormalities, Multiple/genetics
  • Adipose Tissue, Brown/pathology
  • Animals, Outbred Strains
  • Calcinosis/genetics
  • Cardiomyopathies/genetics
  • Catalysis
  • Chylous Ascites/genetics
  • Crosses, Genetic
  • Dimerization
  • Enzyme Induction
  • Genes
  • Genes, Lethal
  • Genotype
  • Germ-Free Life
  • Glucose/metabolism
  • Glucose/pharmacology
  • Hypertrophy
  • Hypoglycemia/genetics
  • Insulin/pharmacology
  • Liver/pathology
  • Mice, Inbred ICR
  • Mice, Knockout
  • Muscle Fibers, Skeletal/pathology
  • Necrosis
  • Phosphatidylinositol 3-Kinases/deficiency
  • Phosphatidylinositol 3-Kinases/physiology
  • Phosphorylation
  • Protein Isoforms/deficiency
  • Protein Isoforms/genetics
  • Protein Isoforms/physiology
  • Protein Processing, Post-Translational/genetics
  • Protein Subunits
  • Second Messenger Systems/genetics
  • B-Lymphocytes/physiology
  • Cell Division/immunology
  • Cell Lineage/immunology
  • Cell Transformation, Neoplastic/metabolism
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors
  • Leukemia/physiopathology
  • Mice, Inbred BALB C
  • Pregnancy
  • Protein Kinases/metabolism
  • Proto-Oncogene Proteins c-abl/genetics
  • Signal Transduction/physiology
  • TOR Serine-Threonine Kinases
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
Strain Development
Mutation was maintained as heteroqygous for approximately 20 generations in mixed background at Harvard and UCLA, then backcrossed 12 generations BALB/c at UCI.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Hematology
  • Immunology and Inflammation
  • Metabolism
Donor
David Fruman, Ph.D., University of California, Irvine
Primary Reference
  • Fruman DA, Snapper SB, Yballe CM, Davidson L, Yu JY, Alt FW, Cantley LC. Impaired B cell development and proliferation in absence of phosphoinositide 3-kinase p85alpha. Science. 1999 Jan 15;283(5400):393-7. (Medline PMID: 9888855)
  • Fruman DA, Mauvais-Jarvis F, Pollard DA, Yballe CM, Brazil D, Bronson RT, Kahn CR, Cantley LC. Hypoglycaemia, liver necrosis and perinatal death in mice lacking all isoforms of phosphoinositide 3-kinase p85 alpha. Nat Genet. 2000 Nov;26(3):379-82. (Medline PMID: 11062485)
  • Kharas MG, Deane JA, Wong S, O'Bosky KR, Rosenberg N, Witte ON, Fruman DA. Phosphoinositide 3-kinase signaling is essential for ABL oncogene-mediated transformation of B-lineage cells. Blood. 2004 Jun 1;103(11):4268-75. Epub 2004 Feb 19. (Medline PMID: 14976048)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
014244-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
014244-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
014244-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.