Strain Detail Sheet

Strain Name    :

STOCK Pik3r1tm2Dfr/Mmucd

Stock Number :

014244-UCD

Other Names   :

Pik3r1-null, STOCK Pik3r1tm2Dfr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Pik3r1tm1Dfr
Name: targeted mutation 1, David A Fruman
Alteration at locus: Targeted Mutation
Gene Symbol: Pik3r1
Name: phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
Chromosome: 13
Alteration at locus: Targeted Mutation

Genetic Alterations:
A neocassette replaces exon 7 and some intronic DNA.

Genotype Determination:

ES Cell Line: TC1/TC-1 derived from 129SvEv x C57BL/6

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Perinatal lethal due to liver necrosis. Other phenotypes include chylous ascites, hypoglycemica, enlarged muscle fibers, impaired erythropoiesis and impaired B cell development. Mice are severely hypoglycemic.

Heterozygous phenotype: Hypoglycemia, increased insulin sensitivy and glucose tolerance. This has been shown in the mixed background but not tested in BalB/C background.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Pik3r1tm2Dfr/Pik3r1tm2Dfr
        either: C.129S6-Pik3r1<tm2Dfr> or (involves: 129S6/SvEvTac * BALB/c * C57BL/6)
  • mortality/aging
    • complete perinatal lethality (MGI Ref ID J:94077)
      • presumed due to liver necrosis
  • homeostasis/metabolism phenotype
    • ascites (MGI Ref ID J:94077)
      • chylous ascites
    • hypoglycemia (MGI Ref ID J:94077)
      • severe hypoglycemia
  • immune system phenotype
    • abnormal B cell differentiation (MGI Ref ID J:94077)
      • development of B lymphocytes is impaired
  • hematopoietic system phenotype
    • abnormal B cell differentiation (MGI Ref ID J:94077)
      • development of B lymphocytes is impaired
    • abnormal erythropoiesis (MGI Ref ID J:94077)
      • erythropoiesis is impaired
  • liver/biliary system phenotype
    • hepatic necrosis (MGI Ref ID J:94077)
  • muscle phenotype
    • increased skeletal muscle fiber size (MGI Ref ID J:94077)

Strain of Origin: 129SvEv x C57Bl/6

Strain genetic background: BalB/C

Strain Development: Mutation was maintained as heteroqygous for approximately 20 generations in mixed background at Harvard and UCLA, then backcrossed 12 genrations to Balb/C at UCI.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Hematology
  • Immunology and Inflammation
  • Metabolism

Strain Origin

Donor: David Fruman, Ph.D., University of California, Irvine

Primary Reference:

  • Fruman DA, Snapper SB, Yballe CM, Davidson L, Yu JY, Alt FW, Cantley LC. Impaired B cell development and proliferation in absence of phosphoinositide 3-kinase p85alpha. Science. 1999 Jan 15;283(5400):393-7. (Medline PMID: 9888855)
  • Fruman DA, Mauvais-Jarvis F, Pollard DA, Yballe CM, Brazil D, Bronson RT, Kahn CR, Cantley LC. Hypoglycaemia, liver necrosis and perinatal death in mice lacking all isoforms of phosphoinositide 3-kinase p85 alpha. Nat Genet. 2000 Nov;26(3):379-82. (Medline PMID: 11062485)
  • Kharas MG, Deane JA, Wong S, O'Bosky KR, Rosenberg N, Witte ON, Fruman DA. Phosphoinositide 3-kinase signaling is essential for ABL oncogene-mediated transformation of B-lineage cells. Blood. 2004 Jun 1;103(11):4268-75. Epub 2004 Feb 19. (Medline PMID: 14976048)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
014244-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
014244-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
014244-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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