Strain Name:
B6.129-Asah2tm1Rlp/Mmnc
Stock Number:
014718-UNC
Citation ID:
RRID:MMRRC_014718-UNC

Gene Information

Asah2tm1Rlp
Name: N-acylsphingosine amidohydrolase 2; targeted mutation 1, Richard L Proia
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
Asah2
Name: N-acylsphingosine amidohydrolase 2
Synonyms: neutral/alkaline ceramidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
NCBI: 54447
VEGA: 19
Homologene: 10310
Genetic Alterations
A neo cassette was inserted between a BamHI site in exon 17 and a MluI site in intron 19 in the same orientation as the Asah2 gene.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    TC1/TC-1 derived from 129S6/SvEvTac
    Phenotype
    Homozygous: The Asah2 KO mice grow normally, do not exhibit obvious abnormalities, are fertile, and are viable for up to one year. This mice are deficient in the intestinal degradation of ceramide.

    Heterozygous:
    The Asah2 KO heterozygous grow normally, do not exhibit obvious abnormalities, are fertile, and are viable for up to one year.
    Mammalian Phenotype Terms
    Allelic Composition: Asah2tm1Rlp/Asah2tm1Rlp (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

    MeSH Terms
    • Alleles
    • Amidohydrolases/genetics
    • Amidohydrolases/metabolism
    • Amidohydrolases/physiology
    • Animals
    • Blotting, Northern
    • Blotting, Western
    • Cell Membrane/metabolism
    • Ceramidases
    • Ceramides/chemistry
    • Ceramides/metabolism
    • Chromatography, Liquid
    • Genetic Vectors
    • Heterozygote
    • Immunohistochemistry
    • Intestine, Small/metabolism
    • Intestines/metabolism
    • Lysophospholipids/chemistry
    • Mass Spectrometry
    • Mice
    • Mice, Inbred C57BL
    • Mice, Transgenic
    • Models, Biological
    • Models, Genetic
    • Neutral Ceramidase
    • Phosphorylation
    • Phosphotransferases (Alcohol Group Acceptor)/metabolism
    • Polymerase Chain Reaction
    • Reverse Transcriptase Polymerase Chain Reaction
    • Signal Transduction
    • Spectrometry, Mass, Electrospray Ionization
    • Sphingolipids/chemistry
    • Sphingolipids/metabolism
    • Sphingomyelins/chemistry
    • Sphingosine/analogs & derivatives
    • Sphingosine/chemistry
    • Time Factors
    • Tissue Distribution
    Strain Development
    The 129/SvEv x C57BL/6 was backcrossed with C57BL/6 for seven generations.
    Suggested Control Mice

    Wildtype littermates

    • Cardiovascular
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Diabetes/Obesity
    • Metabolism
    • Models for Human Disease
    Donor
    Richard L. Proia Ph.D., National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases
    Primary Reference
    Kono M, Dreier JL, Ellis JM, Allende ML, Kalkofen DN, Sanders KM, Bielawski J, Bielawska A, Hannun YA, Proia RL. Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids. J Biol Chem. 2006 Mar 17;281(11):7324-31. Epub 2005 Dec 27. (Medline PMID: 16380386)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    014718-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    014718-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.