Strain Name:
B6.129P2-Gulotm1Mae/Mmucd
Stock Number:
000015-UCD
Citation ID:
RRID:MMRRC_000015-UCD
Other Names:
Gulo, B6.129P2-Gulotm1Mae/Mmcd

Gene Information

Gulotm1Mae
Name: gulonolactone (L-) oxidase; targeted mutation 1, Nobuyo Maeda
Synonyms: guol-, Gulotm1Unc
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Gulo
Name: gulonolactone (L-) oxidase
Synonyms: L-gulono-gamma-lactone oxidase, sfx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
NCBI: 268756
VEGA: 14
HGNC: HGNC:4695
Homologene: 6566
Genetic Alterations
A neo cassette replaced exons 3 and 4 of the L-gulonolactone-g-gene.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous mice are not able to synthesize ascorbic acid, consequently they depend on vitamin C to sustain life, just like humans. With vitamin C supplement in diet or drinking water, homozygotes can reproduce.
Mammalian Phenotype Terms
Allelic Composition: Gulotm1Mae/Gulotm1Mae (Genetic Background: involves: 129 * C57BL/6 )

Allelic Composition: Gulotm1Mae/Gulotm1Mae (Genetic Background: involves: C57BL/6 )

MeSH Terms
  • Animals
  • Antioxidants/metabolism
  • Aorta, Thoracic/enzymology
  • Aorta, Thoracic/pathology
  • Aorta, Thoracic/ultrastructure
  • Ascorbic Acid/administration & dosage
  • Ascorbic Acid/biosynthesis
  • Ascorbic Acid/blood
  • Ascorbic Acid Deficiency/enzymology
  • Ascorbic Acid Deficiency/genetics
  • Cell Division
  • Cholesterol/blood
  • Cholesterol, HDL/blood
  • Diet
  • Elastic Tissue/pathology
  • Elastic Tissue/ultrastructure
  • Female
  • Genotype
  • Homozygote
  • L-Gulonolactone Oxidase
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Microscopy, Electron
  • Muscle, Smooth, Vascular/cytology
  • Mutagenesis, Site-Directed
  • Rats
  • Sugar Alcohol Dehydrogenases/genetics
  • Sugar Alcohol Dehydrogenases/metabolism
Strain Development
Knockout generated on a 129P2 background and moved to the C57BL/6.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Cardiovascular diseases
  • Diabetes
  • Inflammation
  • Bone matrix biology
Donor
Dr. Nobuyo Maeda, University of North Carolina at Chapel Hill
Primary Reference

Maeda N, Hagihara H, Nakata Y, Hiller S, Wilder J, Reddick R. Aortic wall damage in mice unable to synthesize ascorbic acid. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):841-6 (Medline PMID: 10639167)

Colony and Husbandry Information

Homozygotes require vitamin C (ascorbic acid) in either diet or water, ~1.5 mg/day (approximately, 0.33g/L in water).  Homozygous females seldom become pregnant after 7 months of age.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Heterozygous Female x Heterozygous Male
  • Homozygous Female x Homozygous Male
Generation
N10 As of: 9/2001
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 10 months
Average litter size
8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
000015-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
000015-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.