Strain Detail Sheet

Strain Name    :

B6.C3-DctSlt-lt/Mmmh

Stock Number :

000150-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: DctSlt-lt
Name: slaty light
Chromosome: 14
Alteration at locus: Spontaneous Mutation
Gene Symbol: Dct
Name: dopachrome tautomerase
Chromosome: 14
Alteration at locus: Spontaneous Mutation

Genetic Alterations:
Point mutation (transition) in gene.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Mice homozygous for the mutant allele have a medium gray hair coat. Dopachrome tautomerase is one of the three enzymes that function in the melanogenic pathway.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
DctSlt-lt/Dct+
        C3H/HeJ
  • pigmentation phenotype
    • diluted coat color (MGI Ref ID J:27515)
      • non-agouti mice are dark brown, resembling mice that are homozygous for Dctslt or heterozygous DctSlt-lt/Dctslt
      • heterozygotes with agouti are light agouti
  • integument phenotype
    • diluted coat color (MGI Ref ID J:27515)
      • non-agouti mice are dark brown, resembling mice that are homozygous for Dctslt or heterozygous DctSlt-lt/Dctslt
      • heterozygotes with agouti are light agouti
DctSlt-lt/DctSlt-lt
        C3H/HeJ
  • pigmentation phenotype
    • diluted coat color (MGI Ref ID J:27515)
      • mice are dark brown on a nonagouti background
      • when also heterozygous agouti, the coat is light agouti
      • when also homozygous agouti, the coat is yellowish brown
  • integument phenotype
    • diluted coat color (MGI Ref ID J:27515)
      • mice are dark brown on a nonagouti background
      • when also heterozygous agouti, the coat is light agouti
      • when also homozygous agouti, the coat is yellowish brown

Strain of Origin: C3H/HeJ

Strain genetic background: C57BL/6

Strain Development: Spontaneous mutation occurred on the C3H/HeJ. Backcrossed to C57BL/6J over 10 generations.

Suggested Control Mice:

  • C57BL/6

Research Applications

  • Coat Color
  • Cell Biology

Strain Origin

Donor: Dr. ML Lamoreux, Texas A&M University

Primary Reference:

  • Budd PS. Jackson IJ. Structure of the mouse tyrosinase-related protein-2/dopachrome tautomerase (Tyrp2/Dct) gene and sequence of two novel slaty alleles. Genomics. 29(1):35-43, 1995 Sep 1. (Medline PMID: 8530099)
  • Lamoreux ML, Wakamatsu K, Ito S. Interaction of major coat color gene functions in mice as studied by chemical analysis of eumelanin and pheomelanin. Pigment Cell Res. 2001 Feb;14(1):23-31. (Medline PMID:11277491)

Colony and Husbandry Information

Special Considerations

Maintain on a 9% fat diet. Note wean age.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Medium gray

Other:


Breeding

MMRRC Breeding System: Sib mating, with occassional backcross to C57BL/6.

Breeding Scheme(s):

  • Homozygous mutant female x Homozygous mutant male
  • Homozygous mutant female x Homozygous wildtype male
  • Homozygous wildtype female x Homozygous mutant male

Generation: Unknown

Overall Breeding Performance: Good (similar to C57BL/6)

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 26 weeks 26 weeks

Average litter size: Unknown

Recommended wean age: 4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000150-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000150-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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