Strain Name:
B6;129-Rapsntm1Jrs/Mmucd
Stock Number:
015108-UCD
Citation ID:
RRID:MMRRC_015108-UCD
Other Names:
KORAP, B6;129-Rapsntm1Jrs/Mmcd

Strain Information

Rapsntm1Jrs
Name: receptor-associated protein of the synapse; targeted mutation 1, Joshua R Sanes
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
Rapsn
Name: receptor-associated protein of the synapse
Synonyms: Nraps, 43kDa acetylcholine receptor-associated protein, Raps, rapsyn
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knockout
NCBI: 19400
HGNC: HGNC:9863
Homologene: 3708
Genetic Alterations
~1kb of the gene including 119 bp of exon 3 was deleted and replaced with a neo cassette.
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Animals have difficulty breathing and suckling and die a few hours after birth. Postsynaptic acetylcholine receptors fail to aggregate opposite motor nerve terminals.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Rapsntm1Jrs/Rapsntm1Jrs (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

MeSH Terms
  • Animals
  • Axons/physiology
  • Basement Membrane/physiology
  • Cell Line
  • Mice
  • Mice, Transgenic
  • Muscle Proteins/deficiency
  • Muscle Proteins/genetics
  • Muscle Proteins/physiology
  • Mutagenesis
  • Neuromuscular Junction/embryology
  • Neuromuscular Junction/ultrastructure
  • Receptors, Nicotinic/deficiency
  • Receptors, Nicotinic/genetics
  • Receptors, Nicotinic/physiology
  • Synaptic Membranes/physiology
  • Synaptic Membranes/ultrastructure
  • Transcription, Genetic
Strain Development
Chimeras were crossed with C57BL/6 animals to generate heterozygotes. Mutants are perinatal lethal and the line has been maintained by crossing heterozygous siblings.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Medha Gautam, Ph.D., St. Louis University Medical School.
Primary Reference
Gautam M, Noakes PG, Mudd J, Nichol M, Chu GC, Sanes JR, Merlie JP. Failure of postsynaptic specialization to develop at neuromuscular junctions of rapsyn-deficient mice. Nature. 1995 Sep 21;377(6546):232-6. (Medline PMID: 7675108)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti or black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)

Wild-type female x Heterozygous male littermate or reciprocal mating

Overall Breeding Performance
Excellent
Average litter size
6-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
015108-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
015108-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
015108-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.