Strain Detail Sheet

Strain Name    :

B6;129-Rapsntm1Jrs/Mmucd

Stock Number :

015108-UCD

Other Names   :

KORAP, B6;129-Rapsntm1Jrs/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Rapsntm1Jrs
Name: targeted mutation 1, Joshua R Sanes
Alteration at locus: Knockout
Gene Symbol: Rapsn
Name: receptor-associated protein of the synapse
Chromosome: 2
Alteration at locus: Knockout

Genetic Alterations:
~1kb of the gene including 119 bp of exon 3 was deleted and replaced with a neo cassette.

Genotype Determination:

ES Cell Line: R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Animals have difficulty breathing and suckling and die a few hours after birth. Postsynaptic acetylcholine receptors fail to aggregate opposite motor nerve terminals.

Heterozygous phenotype: No obvious phenotype.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Rapsntm1Jrs/Rapsntm1Jrs
        involves: 129S1/Sv * 129X1/SvJ
  • mortality/aging
    • complete perinatal lethality (MGI Ref ID J:28777)
      • animals die within hours of birth
  • nervous system phenotype
    • abnormal neuromuscular synapse morphology (MGI Ref ID J:28777)
      • failure of neuromuscular synapse postsynaptic differentiation (MGI Ref ID J:28777)
        • absence of acetylcholine receptor clusters at end plate band
        • other postsynaptic proteins fail to cluster at synaptic sites
  • muscle phenotype
    • muscle weakness (MGI Ref ID J:28777)
      • animals cannot support themselves on all fours and cannot lift their heads
  • respiratory system phenotype
    • respiratory distress (MGI Ref ID J:28777)
      • animals breathe weakly

Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl<+>

Strain genetic background: B6;129

Strain Development: Chimeras were crossed with C57BL/6 animals to generate heterozygotes. Mutants are perinatal lethal and the line has been maintained by crossing heterozygous siblings.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Medha Gautam, Ph.D., St. Louis University Medical School.

Primary Reference:

Gautam M, Noakes PG, Mudd J, Nichol M, Chu GC, Sanes JR, Merlie JP. Failure of postsynaptic specialization to develop at neuromuscular junctions of rapsyn-deficient mice. Nature. 1995 Sep 21;377(6546):232-6. (Medline PMID: 7675108)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Agouti or black

Other:

Breeding

MMRRC Breeding System: Sib-mating

Breeding Scheme(s):

  • Wild-type female x Heterozygous male littermate or reciprocal mating

Generation:

Overall Breeding Performance: Excellent

Reproductive Statistics

Viability and Fertility: Unknown

Average litter size: 6-9

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
015108-UCD-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
015108-UCD-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
015108-UCD-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 04/17/2014