Strain Name    : C57BL/6J-Ptpn6m1Btlr/Mmucd

Stock Number : 015198-UCD

Other Names   : Spin, C57BL/6-Ptpn6m1Btlr/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Ptpn6m1Btlr
Name: mutation 1, Bruce Beutler
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Ptpn6
Name: protein tyrosine phosphatase, non-receptor type 6
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations:
Y208N in C-SH2 domain of SHP-1

Genotype Determination:

ES Cell Line:

Additional mutations found on this strain:

AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00710:Ptpn6 APN 6 124732356 missense probably benign 0.02
IGL01490:Ptpn6 APN 6 124728344 missense probably damaging 1.00
IGL01865:Ptpn6 APN 6 124732465 missense probably damaging 1.00
IGL02017:Ptpn6 APN 6 124732486 missense possibly damaging 0.90
IGL02272:Ptpn6 APN 6 124721208 missense probably benign 0.26
IGL02276:Ptpn6 APN 6 124728865 missense probably damaging 1.00
IGL02556:Ptpn6 APN 6 124728660 missense probably benign 0.28
spin2 UTSW 6 124732369 missense probably damaging 1.00
R0183:Ptpn6 UTSW 6 124728951 missense probably damaging 1.00
R0254:Ptpn6 UTSW 6 124728150 missense possibly damaging 0.70
R0636:Ptpn6 UTSW 6 124725279 missense probably benign 0.00
R0835:Ptpn6 UTSW 6 124727536 critical splice acceptor site probably null
R1383:Ptpn6 UTSW 6 124721893 missense probably damaging 1.00
R1638:Ptpn6 UTSW 6 124721185 missense probably benign 0.00
R1900:Ptpn6 UTSW 6 124728933 missense probably benign 0.01
R2047:Ptpn6 UTSW 6 124721789 missense probably benign 0.00
R2143:Ptpn6 UTSW 6 124724984 missense probably benign 0.00
R3907:Ptpn6 UTSW 6 124725276 missense probably benign 0.01
R4082:Ptpn6 UTSW 6 124728419 missense not run
R4382:Ptpn6 UTSW 6 124727398 missense not run

Phenotype

Homozygous phenotype: Inflammation of the paws. Viable to at least 12 months age. Splenomegaly. Normal coat.

Heterozygous phenotype: Normal

Mammalian Phenotype Terms:
Allelic Composition: Ptpn6m1Btlr/Ptpn6m1Btlr (Genetic Background: C57BL/6J-Ptpn6m1Btlr/Mmucd )

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: G1 father and G2 mother generated G3 progeny. Breed G3 brother-sister mutants to generate G4 progeny. Breed G4 siblings to generate G5 mutants.

Suggested Control Mice: Wild-type littermates

  • Apoptosis
  • Cancer
  • Cell Biology
  • Dermatology
  • Hematology
  • Immunology and Inflammation
  • Models for Human Disease
  • Virology

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Croker BA; Lawson BR; Berger M; Eidenschenk C; Blasius AL; Moresco EM; Sovath S; Cengia L; Shultz LD; Theofilopoulos AN; Pettersson S; Beutler BA, Inflammation and autoimmunity caused by a SHP1 mutation depend on IL-1, MyD88, and a microbial trigger., Proc Natl Acad Sci U S A 2008 Sep 30;105(39):15028-33 (Medline PMID: 18806225)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
015198-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
015198-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
015198-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.