Strain Name:
B6.129S6(Cg)-Gcktm3Mgn/Mmucd
Stock Number:
015249-UCD
Citation ID:
RRID:MMRRC_015249-UCD
Other Names:
gk A456V, B6.129S6(Cg)-Gcktm3Mgn/Mmcd

Gene Information

Allele Symbol: Gcktm3Mgn (Mus musculus (mouse))
Name: glucokinase; targeted mutation 3, Mark A Magnuson
Synonyms: GK(A456V)
Chromosome: 11
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:3701764
Gene Symbol: Gck (Mus musculus (mouse))
Name: glucokinase
Synonyms: Gls006, HK4, MODY2, hexokinase 4, Hlb62
Chromosome: 11
Alteration at locus: Targeted Mutation
Monarch Initiative (Disease and Phenotype Associations): MGI:1270854
Genetic Alterations
A single base mutation was introduced into exon 10 via site specific mutagenesis to change amino acid 456 from alanine to valine.
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Since the gk A456V mutation enhances the activity of glucokinase these animals are moderately hypoglycemic phenotype. Despite this, they are viable and appear healthy.

Heterozygous: Heterozygous animals, which exhibit milder hypoglycemia, are also viable and appear healthy.
Mammalian Phenotype Terms
Allelic Composition: Gcktm3Mgn/Gcktm3Mgn (Genetic Background: 129S6/SvEvTac-Gcktm3Mgn )

Allelic Composition: Gcktm3Mgn/Gck+ (Genetic Background: 129S6/SvEvTac-Gcktm3Mgn )

Allelic Composition: Gcktm3Mgn/Gck+ (Genetic Background: B6.129S6-Gcktm3Mgn )

MeSH Terms
  • Amino Acid Substitution
  • Animals
  • Blood Glucose/metabolism
  • Carrier Proteins/chemistry
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Enzyme Activation/genetics
  • Enzyme Stability/genetics
  • Glucokinase/chemistry
  • Glucokinase/genetics
  • Glucokinase/metabolism
  • Glucose Metabolism Disorders/enzymology
  • Glucose Metabolism Disorders/genetics
  • Glucose Metabolism Disorders/pathology
  • Hot Temperature
  • Insulin/secretion
  • Liver/enzymology
  • Liver/pathology
  • Mice
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Mutation, Missense
  • Phenotype
  • Protein Binding/genetics
  • Recombinant Proteins/chemistry
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
Strain Development
After achieving germline transmission mice carrying the mutant gk A456V allele were bred to EIIa-Cre transgenic mice in order to delete a neomycin resistance (neoR) cassette. NeoR minus mice were then backcrossed for a total of twelve generations with C57BL/6J mice to obtain a congenic line.
Suggested Control Mice
  • Wildtype littermates
  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease
  • Research Tools
Donor
Mark A. Magnuson, M.D., Vanderbilt University
Primary Reference
Pino MF, Kim KA, Shelton KD, Lindner J, Odili S, Li C, Collins HW, Shiota M, Matschinsky FM, Magnuson MA. Glucokinase thermolability and hepatic regulatory protein binding are essential factors for predicting the blood glucose phenotype of missense mutations. J Biol Chem. 2007 May 4;282(18):13906-16. Epub 2007 Mar 12. (Medline PMID: 17353190)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
015249-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,549.67
Non-Profit / For-Profit
Aliquot Approximate quantity3 : 20-40 embryos / aliquot
015249-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $871.90
Non-Profit / For-Profit
Aliquot Approximate quantity2
015249-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $6,017.01
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.