Strain Name:
B6.Cg-am/Mmmh
Stock Number:
000154-MU
Citation ID:
RRID:MMRRC_000154-MU
Major Collection:

Strain Information

am
Name: nonagouti; mottled
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Radiation Induced
a
Name: nonagouti
Synonyms: agouti, As, ASP, agouti signal protein
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Radiation Induced
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Genetic Alterations
Targeted mutation, radiation induced
Genotype Determination
Phenotype
The mutant allele is recessive to the dominant wildtype allele, and dominant to the recessive wildtype allele. This mutant allele on this background produces a mottled to variable coat color ranging from nonagouti to agouti and differing from mouse to mouse, but on the JU background (MMRRC:000155-MU) the mice are agouti without mottling or variability.

The agouti locus regulations the production of pheomelanin (yellow melanin pigment) versus eumelanin (nonyellow melanin pigment) in closely regulated patterns within individual hairs and over different areas of the body.

Homozygous Agouti-mottled mice (MMRRC:000154) are mottled in a chimeric pattern of agouti and nonagouti.

Mice mutant at the agouti locus offer a remarkably broad range of phenotypic attributes that have been and will in future be useful for the study of obesity, diabetes, and the mechanisms by which genes and their products interact to regulate phenotypes.
Mammalian Phenotype Terms
Allelic Composition: am/a (Genetic Background: involves: 101/Rl * C3H/Rl )

MeSH Terms
  • Agouti Signaling Protein
  • Alleles
  • Animals
  • Base Sequence
  • Chromosome Inversion
  • Chromosome Mapping
  • DNA/genetics
  • Gene Expression Regulation
  • Genes, Recessive
  • Hair Color/genetics
  • Intercellular Signaling Peptides and Proteins
  • Lung Diseases, Interstitial/genetics
  • Lymphoproliferative Disorders/genetics
  • Male
  • Melanins/biosynthesis
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Mutant Strains/genetics
  • Molecular Sequence Data
  • Mutagenesis
  • Phenotype
  • Proteins/genetics
  • RNA, Messenger/genetics
  • Transcription, Genetic
  • Intramolecular Oxidoreductases
  • Isomerases/genetics
  • Isomerases/metabolism
  • Melanins/metabolism
  • Melanocytes/metabolism
  • Membrane Glycoproteins
  • Models, Biological
  • Molecular Weight
  • Monophenol Monooxygenase/genetics
  • Monophenol Monooxygenase/metabolism
  • Mutation
  • Oxidoreductases
  • Proteins/metabolism
  • RNA, Messenger/metabolism
  • Skin/metabolism
  • 5-Methylcytosine
  • Betaine/metabolism
  • Choline/metabolism
  • Cytosine/analogs & derivatives
  • Cytosine/metabolism
  • Dietary Supplements
  • Female
  • Folic Acid/metabolism
  • Genomic Imprinting
  • Methionine/metabolism
  • Mice, Inbred Strains
  • Pregnancy
  • S-Adenosylmethionine/metabolism
  • Vitamin B 12/metabolism
  • Zinc/metabolism
  • Genes
  • Homozygote
  • Melanins/physiology
  • Skin Physiological Phenomena
  • Skin Transplantation
  • Species Specificity
  • Transplantation, Homologous
Strain Development
The original mutant was the offspring of an irradiated (101/Rl x C3H/Rl)F1 mouse that had been crossed to the T stock (homozygous for 7 recessive markers, but not inbred). The stock was then either brother-sister mated or backcrossed onto the C57BL/10 background (exact breeding information to determine which mating program was performed is not available). These mice were backcrossed onto C57BL/6J by Linda Siracusa and then transferred to Lynn Lamoreux, where they were maintained by sib mating and occassional backcrosses onto C57BL/6J.
Suggested Control Mice
  • C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Pigmentation
  • Developmental Biology
  • Gene Regulation
  • Signaling Molecules
  • Pattern formation
  • Cancer incidence
  • Obesity
  • Diabetes
Donor
Dr. Lynn Lamoreux, Texas A&M University
Primary Reference
  • Hustad CM, Perry WL, Siracusa LD, Rasberry C, Cobb L, Cattanach BM, Kovatch R, Copeland NG, Jenkins NA. Molecular genetic characterization of six recessive viable alleles of the mouse agouti locus.Genetics. 1995 May;140(1):255-65. (Medline PMID: 7635290)
  • Lamoreux ML, Zhou BK, Rosemblat S, Orlow SJ. The pinkeyed-dilution protein and the eumelanin/pheomelanin switch: in support of a unifying hypothesis. Pigment Cell Res. 1995 Oct;8(5):263-70. (Medline PMID: 8789201)
  • Wolff GL, Kodell RL, Moore SR, Cooney CA. Maternal epigenetics and methyl supplements affect agouti gene expression in Avy/a mice. FASEB J. 1998 Aug;12(11):949-57. (Medline PMID: 9707167)
  • Poole TW. Dermal-epidermal interactions and the action of alleles at the agouti locus in the mouse. II. The viable yellow (Avy) and mottled agouti (am) alleles. Developmental Biology. 80(2):495-500, 1980 Dec. (Medline PMID: 7004955)
  • Russell LB, Genetic and Functional Mosaicism in the Mouse, Pages 153-81 in Locke M, ed., The Role of the Chromosomes in Development. 1964. Academic Press, New York. (Medline PMC: 1468546)

Colony and Husbandry Information

Feed breeding mice 9% fat diet.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Mottled agouti
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
Homozygous female x Homozygous male
Generation
N10+F20+
Overall Breeding Performance
Good
Average litter size
7
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000154-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000154-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000154-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.