Strain Detail Sheet

Strain Name:
C.129P2(Cg)-Igh-8tm1Schr/Mmucd
Stock Number:
015506-UCD
Citation ID:
RRID:MMRRC_015506-UCD
Other Names:
C.129(B6)-Igg3tm1 N10 (IgG3 KO), C.129P2(Cg)-Igh-8tm1Schr/Mmcd

Strain Information

Gene Details

Igh-8tm1Schr
Name: immunoglobulin heavy chain 8 (heavy chain of IgG3); targeted mutation 1, John R Schreiber
Synonyms: Igh-8tm1Sch, IgG3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Igh-8
Name: immunoglobulin heavy chain 8 (heavy chain of IgG3)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Genetic Alterations
A total of 54 bp of the exon encoding the CH1 domain were replaced in a murine gamma 3 allele heavy chain allele with a targeting vector containing the diphtheria toxin gene and a neomycin-resistance cassette (neor) bearing a beta-actin promoter.
Genotype Determination

Genotyping Protocol

ES Cell Line
E14 derived from 129P2/OlaHsd

Strain Description

Phenotype
Homozygous: The homozygous gamma 3 -/- mice lack serum IgG3 (at detectable levels). They appear to be more susceptible to infection with type 3 Streptococcus pneumomoniae.

Heterozygous: The heterozygotes are more similar to the wild-type mice. They produce serum IgG3.
Mammalian Phenotype Terms
Allelic Composition: Igh-8tm1Schr/Igh-8tm1Schr (Genetic Background: C.129P2(BKSW)-Igh-8tm1Schr )
Allelic Composition: Igh-8tm1Schr/Igh-8tm1Schr (Genetic Background: involves: 129P2/OlaHsd * Black Swiss )
MeSH Terms
  • Animals
  • Antibodies, Bacterial/biosynthesis
  • Antibodies, Bacterial/classification
  • Bacterial Vaccines/immunology
  • Cells, Cultured
  • Crosses, Genetic
  • Female
  • Genes, Immunoglobulin/genetics
  • IgG Deficiency/genetics
  • IgG Deficiency/immunology
  • Immunoglobulin Class Switching/genetics
  • Immunoglobulin G/biosynthesis
  • Immunoglobulin G/genetics
  • Immunoglobulin Heavy Chains/biosynthesis
  • Immunoglobulin Heavy Chains/genetics
  • Lipopolysaccharides/pharmacology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Weight
  • Polysaccharides, Bacterial/immunology
  • Pseudomonas aeruginosa/immunology
  • Recombination, Genetic
  • Spleen/cytology
  • Spleen/immunology
  • Vaccines, Conjugate/immunology
  • Alleles
  • Antibodies, Bacterial/genetics
  • Bacterial Capsules
  • Bacterial Proteins/administration & dosage
  • Bacterial Proteins/immunology
  • Genes, Immunoglobulin
  • Genetic Predisposition to Disease
  • Genotype
  • IgA Deficiency/genetics
  • IgA Deficiency/immunology
  • Immune Sera/administration & dosage
  • Immunization, Passive
  • Immunoglobulin G/classification
  • Injections, Intraperitoneal
  • Opsonin Proteins/pharmacology
  • Phenotype
  • Pneumonia, Pneumococcal/genetics
  • Pneumonia, Pneumococcal/immunology
  • Pneumonia, Pneumococcal/mortality
  • Pneumonia, Pneumococcal/prevention & control
  • Polysaccharides, Bacterial/administration & dosage
  • Sepsis/genetics
  • Sepsis/immunology
  • Sepsis/mortality
  • Streptococcus pneumoniae/growth & development
  • Streptococcus pneumoniae/immunology
  • Vaccines, Conjugate/administration & dosage
Strain Development
This line was created by gene targeting with a neomycin-selectable gamma 3 heavy chain gene vector originating from a lambda129SV genomic library. The targeting vector was then introduced into E14-1 ES cells. The targeted ES cells were injected into C57BL/6 blastocysts and these blastocysts were transferred to the uteri of pseudopregnant C3HB16 females. Resulting males (agouti/C57BL) expressing a chimeric coat color (.25% agouti/chinchilla agouti color) were bred to National Institutes of Health Swiss Black mates. Germline chimeras were defined as producing progeny with an agouti coat color. Male and female agouti littermates (heterozygous agouti/C57BL/Swiss) were then crossed to obtain homozygous knockout (KO) pups. Homozygous wild-type (WT) littermates were saved and bred as controls.

After some time, mice bearing the defective gamma 3 heavy chain allele were backcrossed for 10 generations to BALB/c mice. These mice were then inbred as Line 632.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation
  • Models for Human Disease

Strain Origin

Donor
Neil S. Greenspan, M.D., Ph.D., Case Western Reserve University.
Primary Reference
  • Shapiro DA, Threadgill DS, Copfer MJ, Corey DA, McCool TL, McCormick LL, Magnuson TR, Greenspan NS, Schreiber JR. Gamma 3 gene-disrupted mice selectively deficient in the dominant IgG subclass made to bacterial polysaccharides undergo normal isotype switching after immunization with polysaccharide-protein conjugate vaccines. J Immunol. 1998 Oct 1;161(7):3393-9. (Medline PMID: 9759856)
  • McLay J, Leonard E, Petersen S, Shapiro D, Greenspan NS, Schreiber JR. Gamma 3 gene-disrupted mice selectively deficient in the dominant IgG subclass made to bacterial polysaccharides. II. Increased susceptibility to fatal pneumococcal sepsis due to absence of anti-polysaccharide IgG3 is corrected by induction of anti-polysaccharide IgG1. J Immunol. 2002 Apr 1;168(7):3437-43. (Medline PMID: 11907102)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
White

Breeding

MMRRC Breeding System
Backcross or Sib-mating
Breeding Scheme(s)
  • Backcross: confirm there are no reproduction issues
    • Wild-type (BALB/c) female x Heterozygous male or reciprocal mating
    • Wild-type female x Heterozygous male littermate or reciprocal mating
    • Homozygous female x Homozygous male littermate
Generation
N10

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Not specified Not specified
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
015506-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
015506-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
015506-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.