Strain Name:
B6.Cg-at-Lm Tyrc-ch/Mmmh
Stock Number:
000157-MU
Citation ID:
RRID:MMRRC_000157-MU
Major Collection:

Strain Information

Tyrc-ch
Name: tyrosinase; chinchilla
Synonyms: cr, cch
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Spontaneous Mutation
at-Lm
Name: nonagouti; black and tan Lamoreux
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Spontaneous Mutation
a
Name: nonagouti
Synonyms: agouti, As, ASP, agouti signal protein
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Spontaneous Mutation
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Tyr
Name: tyrosinase
Synonyms: Oca1, skc35
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Spontaneous Mutation
NCBI: 22173
Homologene: 30969
Genetic Alterations
Spontaneous mutations.
Genotype Determination
Phenotype
Mice are darker gray dorsally and light cream colored ventrally

Characteristics of the albino (Tyr) locus encodes the rate-limiting enzyme for pigmentation, which is TYROSINASSE. The absolute requirement for functional TYROSINASE to catalyze the formation of melanin pigment is illustrated by the phenotypes of mice that lack this locus as a result of deletion, for example mice heterozygous for two overlapping deletions, MMRRC:009964 and MMRRC:009965 in which the albino locus is absent. The mice also lack all melanin pigment. Vertebrates that lack normal tyrosinase are characterized by reduction or absence of pigmentation in the eyes and/or skin. If the reduction of pigmentation is severe, optic nerve development may be abnormal, resulting in blindness. Reduction of melanogenesis may result from failure of normal tyrosinase function caused by its abnormal interaction with other proteins, from defects in its regulatory region, from absence or defect of the structural gene. The series of natural mutations that affect availability of normal tyrosinase function includes mutants of this type. C57BL/6J-Tyrc-2J/Tyrc-2J, available at The Jackson Laboratory, lacks tyrosinase activity as a result of point mutation in the structural region of the gene; Acromelanic (MMRRC:000140) may have a similar defect. Extreme dilution (MMRRC:000141) seems to have nearly normal pigmentation in the eyes, but their hair pigmentation is a uniform gray. In dark-eyed-albino (MMRRC:000139) the eye pigmentation is somewhat reduced, but intense, while the hair is unpigmented. Chinchilla mice(MMRRC:000144) are very dark, nearly black. Two of the mutant alleles, chinchilla mottled (MMRRC:000145) and extreme dilution mottled (MMRRC:000142) result in chimerism of hair and eye pigmentation that has been attributed to a rearrangement of the DNA 5kb upstream of the tyrosinase locus. One of the genes that regulates the activity of TYROSINASE is MITF. And there are functional interactions between TYROSINASE and TYROSINASE-RELATED PROTEIN within the pigment cell.
Mammalian Phenotype Terms
Allelic Composition: Tyrc-ch/Tyrc-ch (Genetic Background: involves: C57BL/6 )

Allelic Composition: Tyrc-ch/Tyrc-ch38H (Genetic Background: involves: 101/H * C3H/HeH )

Allelic Composition: Tyrc-42H/Tyrc-ch (Genetic Background: involves: 101/H * C3H/HeH )

Allelic Composition: Tyrc-43H/Tyrc-ch (Genetic Background: involves: 101/H * C3H/HeH )

Allelic Composition: Tyrc-112K/Tyrc-ch (Genetic Background: involves: 101/R1 * C3Hf/R1 * St.2A )

Allelic Composition: Tyrc-40H/Tyrc-ch (Genetic Background: involves: C3H/HeH )

Allelic Composition: Tyrc-a/Tyrc-ch (Genetic Background: involves: C3H/HeJ )

  • pigmentation
  • integument
Allelic Composition: Tyrcm1OR/Tyrc-ch (Genetic Background: involves: C3H/Rl )

MeSH Terms
  • Albinism/genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Catechol Oxidase/genetics
  • Cloning, Molecular
  • DNA/isolation & purification
  • Melanocytes/enzymology
  • Melanocytes/physiology
  • Mice
  • Mice, Inbred C57BL/genetics
  • Mice, Mutant Strains/genetics
  • Molecular Sequence Data
  • Monophenol Monooxygenase/genetics
  • Monophenol Monooxygenase/isolation & purification
  • Mutation
  • Polymorphism, Restriction Fragment Length
  • Alleles
  • DNA/genetics
  • DNA Mutational Analysis
  • Gene Rearrangement
  • Melanins/biosynthesis
  • Melanins/genetics
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • RNA, Messenger/genetics
  • Recombination, Genetic
  • Repetitive Sequences, Nucleic Acid
  • Skin Pigmentation/genetics
  • Skin Pigmentation/physiology
  • Transcription, Genetic
Strain Development
The black and tan agouti mutation arose spontaneously in a colony of mice homozygous for the chinchilla mutation. Both mutations were backcrossed onto the C57BL/6J strain and the chinchilla mutation was bred to homozygosity. The black and tan allele is dominant to the non-agouti allele of the gene and therefore homozygous mice are not distinguishable from the heterozygous mice. The colony has not been bred to homozygosity for the black and tan mutation.
Suggested Control Mice
  • Wild-type littermates
  • C57BL/6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Pigmentation
  • Developmental Biology
  • Gene Regulation
  • Signaling molecules
  • Pattern formation
  • Cancer incidence
  • Obesity
  • Diabetes
Donor
Lynn Lamoreux, Ph.D., Texas Agricultural and Mechanical University.
Primary Reference
Lamoreux ML, Russell ES. Developmental interaction in the pigmentary system of mice. I. Interactions between effects of genes on color of pigment and on distribution of pigmentation in the coat of the house mouse (Mus musculus). J Hered. 1979 Jan-Feb;70(1):31-6. (Medline PMID: 469221)

Kwon BS, Halaban R, Chintamaneni C. Molecular basis of mouse Himalayan mutation. Biochem Biophys Res Commun. 1989 May 30;161(1):252-60. PMID: 2567165 (Medline PMID: 2567165)

Bultman SJ, Klebig ML, Michaud EJ, Sweet HO, Davisson MT, Woychik RP. Molecular analysis of reverse mutations from nonagouti (a) to black-and-tan (a(t)) and white-bellied agouti (Aw) reveals alternative forms of agouti transcripts. Genes Dev. 1994 Feb 15;8(4):481-90. PMID: 8125260 (Medline PMID: 8125260)

Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice.Pigment Cell Res. 1999 Dec;12(6):383-90. (Medline PMID: 10614578)

Colony and Husbandry Information

Feed 9% fat breeder chow

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black and tan carrier mice have a dark gray dorsal coat color with a light cream colored ventrum. Non-carrier black and tan allele mice are dark gray with the same coat color as MMRRC:144.
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
Black and tan female (heterozygous and homozygous genotype is not distinguishable so black and tan mice may be either heterozygous or homozygous at the non-agouti locus and is homozygous for the chinchilla allele) to a black and tan male
Generation
N10+ (C57BL/6J), F5+
Overall Breeding Performance
Good
Average litter size
6-8
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000157-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000157-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000157-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.