Loading Mouse GIF
Loading...

Strain Name:
B6.129X1-Gt(ROSA)26Sortm2(ECFP)Cos/Mmnc
Stock Number:
015748-UNC
Citation ID:
RRID:MMRRC_015748-UNC

Strain Information

Gt(ROSA)26Sortm2(ECFP)Cos
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Frank Costantini
Synonyms: R26R-ECFP, ROSA-stopflox-CFP, R26RCFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
NCBI: 14910
ECFP
Name: enhanced cyan fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Alteration at locus: Targeted Mutation
Genetic Alterations
ECFP cDNA was inserted into the ROSA26 locus, preceded by a loxP-flanked stop sequence.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    JM-1 derived from 129X1/SvJ
    Phenotype
    Homozygous: Same as wild-type

    Heterozygous: Same as wild-type
    Mammalian Phenotype Terms
    Allelic Composition: Gt(ROSA)26Sortm2(ECFP)Cos/Gt(ROSA)26Sor+ (Genetic Background: involves: 129X1/SvJ )

    MeSH Terms
    • Animals
    • Bacterial Proteins/biosynthesis
    • Bacterial Proteins/genetics
    • Brain/cytology
    • Brain/metabolism
    • Cell Line
    • Embryo, Mammalian
    • Female
    • Gene Targeting/methods
    • Genes, Reporter/genetics
    • Genetic Markers/genetics
    • Genetic Vectors
    • Green Fluorescent Proteins
    • Integrases/genetics
    • Luminescent Proteins/biosynthesis
    • Luminescent Proteins/genetics
    • Mice
    • Mice, Inbred C57BL
    • Mice, Inbred Strains
    • Mice, Transgenic
    • Mutagenesis, Insertional
    • Proteins/genetics
    • RNA, Untranslated
    • Recombination, Genetic/genetics
    • Stem Cells/chemistry
    • Stem Cells/metabolism
    • Transfection
    • Viral Proteins/genetics
    Strain GQC Summary
    Gene Specific Genotyping:

    To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

    Background Genetic Quality:

    The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

    Suggested Control Mice
    Littermates of all relevant genotypes.
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Hematology
    • Immunology and Inflammation
    • Internal/Organ
    • Neurobiology
    • Reproduction
    • Research Tools
    Donor
    Frank Costantini, Ph.D., Columbia University Medican Center
    Primary Reference
    Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol. 2001;1:4. Epub 2001 Mar 27. (Medline PMID: 11299042)
    Strain Development
    JM-1 ES cells derived from strain 129X1/SvJ were injected into C57BL/6J blastocysts. The chimeras were bred to C57BL/6J, followed by backcrossing to C57BL/6J for >5 generations before intercrossing to produce homozygotes.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Breeding Scheme(s)

    1 female X 1 male

    Generation
    N5+ (C57BL/6J), F?
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Recommended wean age
    3 weeks

    Order Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    015748-UNC-SPERM Cryo-preserved spermatozoa $520.00 / Non-Profit Aliquot Approximate quantity3
    015748-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.