Strain Name:
B6.129X1-Gt(ROSA)26Sortm2(ECFP)Cos/Mmnc
Stock Number:
015748-UNC
Citation ID:
RRID:MMRRC_015748-UNC

Strain Information

Gt(ROSA)26Sortm2(ECFP)Cos
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Frank Costantini
Synonyms: R26RCFP, ROSA-stopflox-CFP, R26R-ECFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: beta geo, R26, Gtrosa26, Thumpd3as1, Gtrgeo26, ROSA26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
NCBI: 14910
ECFP
Name: enhanced cyan fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Alteration at locus: Targeted Mutation
Genetic Alterations
ECFP cDNA was inserted into the ROSA26 locus, preceded by a loxP-flanked stop sequence.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    JM-1 derived from 129X1/SvJ
    Phenotype
    Homozygous: Same as wild-type

    Heterozygous: Same as wild-type
    Mammalian Phenotype Terms
    Allelic Composition: Gt(ROSA)26Sortm2(ECFP)Cos/Gt(ROSA)26Sor+ (Genetic Background: involves: 129X1/SvJ )

    MeSH Terms
    • Animals
    • Bacterial Proteins/biosynthesis
    • Bacterial Proteins/genetics
    • Brain/cytology
    • Brain/metabolism
    • Cell Line
    • Embryo, Mammalian
    • Female
    • Gene Targeting/methods
    • Genes, Reporter/genetics
    • Genetic Markers/genetics
    • Genetic Vectors
    • Green Fluorescent Proteins
    • Integrases/genetics
    • Luminescent Proteins/biosynthesis
    • Luminescent Proteins/genetics
    • Mice
    • Mice, Inbred C57BL
    • Mice, Inbred Strains
    • Mice, Transgenic
    • Mutagenesis, Insertional
    • Proteins/genetics
    • RNA, Untranslated
    • Recombination, Genetic/genetics
    • Stem Cells/chemistry
    • Stem Cells/metabolism
    • Transfection
    • Viral Proteins/genetics
    Strain Development
    JM-1 ES cells derived from strain 129X1/SvJ were injected into C57BL/6J blastocysts. The chimeras were bred to C57BL/6J, followed by backcrossing to C57BL/6J for >5 generations before intercrossing to produce homozygotes.
    Suggested Control Mice

    Wild-type littermates

    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on two XY sample,s NR3313 and UY8135, at the time of importation and the conclusions are consistent in all samples. The results of the analysis are congruent with the SDS and NR3313 is the representative sample selected for the report. The primary (host) background is C57BL/6J. The secondary (donor) background is consistent with 129X1/SvJ, which is indicated in the submission and the SDS as the donor strain. The mitochondrial genome is consistent with both the host and donor background (identical by descent). The Y chromosome is inconsistent with at least one backcross to a male from the host background. The region containing the allele of interest has the expected donor background (129X1/SvJ). Both sample are homozygous for the donor background at the locus. The following constructs are detected in all samples: bpA and g_FP. g_FP probes detect the expected CFP construct.

    The genome of MMRRC: 015748 mice strain is 96.6 % inbred and 99.2% congenic at the strain level. 3.4% of the genome is segregating for the secondary (donor) background. The level of congenicity estimated by miniMUGA is consistent with the number of backcross generations reported in the SDS.

    The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible.

    MiniMUGA Sample Report
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Hematology
    • Immunology and Inflammation
    • Internal/Organ
    • Neurobiology
    • Reproduction
    • Research Tools
    Donor
    Frank Costantini, Ph.D., Columbia University Medican Center
    Primary Reference
    Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, Costantini F. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol. 2001;1:4. Epub 2001 Mar 27. (Medline PMID: 11299042)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Breeding Scheme(s)

    1 female X 1 male

    Generation
    N5+ (C57BL/6J), F?
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    015748-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    015748-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.