Strain Name    : B6;CBA-Tg(Axin2-d2EGFP)5Cos/Mmnc

Stock Number : 015749-UNC

Gene Information

(provided by MGI)
Allele Symbol: Tg(Axin2-d2EGFP)5Cos
Name: transgene insertion 5, Frank Costantini
Alteration at locus: Transgenic
Promoter: Axin2
Name: axin2
Alteration at locus: Transgenic
Reporter: d2EGFP (Jellyfish)
Name:
Alteration at locus: Transgenic
Genetic Alterations:
Axin2 regulatory elements (promoter and first intron) were joined to d2EGFP (a form of EGFP with a short half-life, Clontech), and vector sequences were removed before microinjection to generate transgenic lines.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line:

    Phenotype

    Homozygous phenotype: none except expression of d2EGFP

    Hemizygous phenotype: none except expression of d2EGFP

    Mammalian Phenotype Terms:

    Strain of Origin: C57BL/6 x CBAF2

    Strain genetic background: C57BL/6J

    Strain Development: Transgenic mouse derived by microinjection into B6CBAF1 x B6CBAF1 zygote. Maintained by backcross to C57BL/6.

    Suggested Control Mice:

    • Wildtype littermates

    • Cancer
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Internal/Organ
    • Neurobiology
    • Research Tools

    Donor: Frank Costantini, Ph.D., Columbia University Medical Center

    Primary Reference:

    Jho EH, Zhang T, Domon C, Joo CK, Freund JN, Costantini F. Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway. Mol Cell Biol. 2002 Feb;22(4):1172-83. (Medline PMID: 11809808)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Coat color:

    Other:

    MMRRC Breeding System:

    Breeding Scheme(s):

    Generation: Donating Investigator submitted mice that had been backcrossed 3-4 generations

    Overall Breeding Performance:

    Breeding Comments: Backcross to C57BL/6

    Viability and Fertility: Unknown

    Average litter size:

    Recommended wean age:

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    015749-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.