Strain Name:
MRL.Cg-Igh-8tm1Schr Faslpr/Mmmh
Stock Number:
015868-MU
Citation ID:
RRID:MMRRC_015868-MU

Strain Information

Igh-8tm1Schr
Name: immunoglobulin heavy chain 8 (heavy chain of IgG3); targeted mutation 1, John R Schreiber
Synonyms: Igh-8tm1Sch, IgG3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Igh-8
Name: immunoglobulin heavy chain 8 (heavy chain of IgG3)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Faslpr
Name: Fas cell surface death receptor; lymphoproliferation
Synonyms: Tnfrf6lpr, Fas-def, lpr, MRL/lpr, Fas-, Tnfrsf6lpr, Tnfrsf6lpr
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Spontaneous Mutation
Fas
Name: Fas cell surface death receptor
Synonyms: Tnfrsf6, APO-1, TNFR6, CD95
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Spontaneous Mutation
NCBI: 14102
VEGA: 19
Homologene: 27
Genetic Alterations
A total of 54 bp of the exon encoding the CH1 domain were replaced in a murine gamma 3 allele heavy chain allele with a targeting vector containing the diphtheria toxin gene and a neomycin-resistance cassette (neor) bearing a beta-actin promoter.
Genotype Determination
ES Cell Line
E14
Phenotype
Homozygous: The homozygous gamma 3 -/- mice lack serum IgG3 (at detectable levels). On average, they live longer than gamma 3 +/- or +/+ mice (of the same background) and exhibit less progression of spontaneous lupus-like renal disease as assessed by both functional criteria and renal histopathology.

Heterozygous: The heterozygotes are more similar to the wild-type mice in many respects. They produce serum IgG3 and show disease manifestations more similar to +/+ than to -/- mice.
Mammalian Phenotype Terms
Allelic Composition: Igh-8tm1Schr/Igh-8tm1Schr (Genetic Background: C.129P2(BKSW)-Igh-8tm1Schr )

Allelic Composition: Igh-8tm1Schr/Igh-8tm1Schr (Genetic Background: involves: 129P2/OlaHsd * Black Swiss )

Allelic Composition: Faslpr/Faslpr (Genetic Background: AK.MRL-Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: B6.Cg-Apoetm1Unc Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: B6.MRL-Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: B6.MRL-Faslpr/J )

Allelic Composition: Faslpr/Faslpr (Genetic Background: C3.MRL-Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: C3.MRL-Faslpr/J )

Allelic Composition: Faslpr/Faslpr (Genetic Background: involves: 129P2/OlaHsd * MRL )

Allelic Composition: Faslpr/Faslpr (Genetic Background: involves: C3H * MRL/Mp )

Allelic Composition: Faslpr/Faslpr (Genetic Background: involves: C57BL/6 * MRL/Mp )

Allelic Composition: Faslpr/Faslpr (Genetic Background: involves: MRL/Mp )

Allelic Composition: Faslpr/Faslpr (Genetic Background: MRL-Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: MRL.Cg-Irf1tm1Mak Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: MRL.Cg-Tnfrsf9tm1Byk Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: MRL/Mp-Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: MRL/Mp-Faslpr atms )

Allelic Composition: Faslpr/Faslpr (Genetic Background: MRL/MpJ-Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: MRL/MpJ-Faslpr/J )

Allelic Composition: Faslpr/Faslpr (Genetic Background: NOD.MRL(B6)-Faslpr )

Allelic Composition: Faslpr/Faslpr (Genetic Background: NOD.MRL-Faslpr )

Allelic Composition: Faslpr/Fastm1.1Cgn (Genetic Background: involves: C57BL/6 * MRL )

Strain Development
The original IgG3-deficient mouse line was created by gene targeting with a neomycin-selectable gamma 3 heavy chain gene vector originating from a lambda129SV genomic library. The targeting vector was then introduced into E14-1 ES cells. The targeted ES cells were injected into C57BL/6 blastocysts and these blastocysts were transferred to the uteri of pseudopregnant C3HB16 females. Resulting males (agouti/C57BL) expressing a chimeric coat color (.25% agouti/chinchilla agouti color) were bred to National Institutes of Health Swiss Black mates. Germline chimeras were defined as producing progeny with an agouti coat color. Male and female agouti littermates (heterozygous agouti/C57BL/Swiss) were then crossed to obtain homozygous knockout (KO) pups. Homozygous wild-type (WT) littermates were saved and bred as controls.

After some time, mice bearing the defective gamm 3 heavy chain allele were backcrossed for 10 generations to BALB/c mice. These mice were then inbred as Line 632. Mice from Line 632 were then used to initiate 12 generations of backcrossing to MRL/MpJ-Tnfrsf6 (MRL/lpr) mice.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Dermatology
  • Hematology
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Neil S. Greenspan, M.D., Ph.D., Case Western Reserve University

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
015868-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
015868-MU-RESUS Litter recovered from cryo-archive $2,028.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.