Strain Name:
FVB.129X1(Cg)-Kcnj1tm1Ges/Mmmh
Stock Number:
015869-MU
Citation ID:
RRID:MMRRC_015869-MU

Strain Information

Kcnj1tm1Ges
Name: potassium inwardly-rectifying channel, subfamily J, member 1; targeted mutation 1, Gary E Shull
Synonyms: ROMK(-), Romk-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Kcnj1
Name: potassium inwardly-rectifying channel, subfamily J, member 1
Synonyms: Kir1.1, ROMK-2, ROMK
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knockout
NCBI: 56379
VEGA: 9
HGNC: HGNC:6255
Homologene: 56764
Genetic Alterations
The neomycin resistance gene was inserted into the large core exon between codons 181 and 182.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: Loss of NaCl absorption in the renal thick ascending limb, hydronephrosis. Viability is poor with approximately half of the homozygotes dying before weaning. After that they appear healthy and survive well.

Heterozygous: same as wildtype
Mammalian Phenotype Terms
Allelic Composition: Kcnj1tm1Ges/Kcnj1tm1Ges (Genetic Background: involves: 129X1/SvJ * Black Swiss )

MeSH Terms
  • Animals
  • Bartter Syndrome/genetics
  • Base Sequence
  • Body Weight
  • DNA Primers
  • Disease Models, Animal
  • Gene Library
  • Genotype
  • Glomerular Filtration Rate/genetics
  • Humans
  • Kidney/pathology
  • Kidney/physiology
  • Kidney/physiopathology
  • Loop of Henle/metabolism
  • Loop of Henle/pathology
  • Loop of Henle/physiology
  • Mice
  • Mice, Knockout
  • Phenotype
  • Polymerase Chain Reaction
  • Sodium Chloride/metabolism
  • Tail
Strain Development
The original mixed background mice were backcrossed onto FVB/N mice
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Internal/Organ
  • Models for Human Disease
  • Renal physiology
Donor
Gary E. Shull, Ph.D., University of Cincinnati College of Medicine
Primary Reference
Lorenz JN, Baird NR, Judd LM, Noonan WT, Andringa A, Doetschman T, Manning PA, Liu LH, Miller ML, Shull GE. Impaired renal NaCl absorption in mice lacking the ROMK potassium channel, a model for type II Bartter's syndrome. J Biol Chem. 2002 Oct 4;277(40):37871-80. Epub 2002 Jul 16. (Medline PMID: 12122007)

Colony and Husbandry Information

Donor breed heterozygotes and included Napa nector in the bottom of the cage. Approximately half of the homozygotes die before weaning but after they are weaned they have normal survival. For maintenance of the line, heterozygotes are used.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
015869-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
015869-MU-RESUS Litter recovered from cryo-archive $2,697.00 / $5,488.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.