Strain Detail Sheet

Strain Name    :

B6;129S-Crlf2tm1Wjl/Mmnc

Stock Number :

015980-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Crlf2tm1Wjl
Name: targeted mutation 1, Warren J Leonard
Alteration at locus: Knockout
Gene Symbol: Crlf2
Name: cytokine receptor-like factor 2
Chromosome: 5
Alteration at locus: Knockout

Genetic Alterations:
TSLPR genomic DNA was obtained from a P1 clone prepared from 129sv mice (Genome Research). The targeting construct was designed to delete all exons of the TSLPR gene and replace them with the neomycin resistance gene (Neo; see Fig. 1) JEM, Volume 200, Number 2, 159-168

Genotype Determination:

ES Cell Line: Not specified derived from 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Connot develop asthma

Heterozygous phenotype:


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Crlf2tm1Wjl/Crlf2tm1Wjl
        involves: 129 * C57BL/6
  • hematopoietic system phenotype
    • abnormal lymphopoiesis (MGI Ref ID J:108209)
      • after sublethal irradiation, mutants do not recover thymic or splenic cellularity after 4 weeks, while controls show high recovery of cellularity after 4 weeks
    • decreased B cell number (MGI Ref ID J:108209)
      • spleens of irradiated mutants have reduced numbers of B220+ CD19+ B cells compared to wild-type controls, indicating less efficient B cell recovery
    • decreased single-positive T cell number (MGI Ref ID J:108209)
      • after irradiation, CD4+ and CD8+ subpopulations in the thymus and spleen are lower in mutants compared to wild-type littermates
  • immune system phenotype
    • abnormal lymphopoiesis (MGI Ref ID J:108209)
      • after sublethal irradiation, mutants do not recover thymic or splenic cellularity after 4 weeks, while controls show high recovery of cellularity after 4 weeks
    • decreased B cell number (MGI Ref ID J:108209)
      • spleens of irradiated mutants have reduced numbers of B220+ CD19+ B cells compared to wild-type controls, indicating less efficient B cell recovery
    • decreased single-positive T cell number (MGI Ref ID J:108209)
      • after irradiation, CD4+ and CD8+ subpopulations in the thymus and spleen are lower in mutants compared to wild-type littermates


The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Crlf2tm1Wjl/Crlf2tm1Wjl
        involves: BALB/c
  • i mmune system phenotype
    • abnormal T cell proliferation (MGI Ref ID J:107431)
      • CD4+ T cells isolated from OVA-immunized mice show less proliferation in response to secondary antigen exposure than wild-type T cells
    • abnormal cytokine secretion (MGI Ref ID J:107431)
      • knockouts sensitized to OVA and challenged with OVA do not show increased levels of cytokines like control mice do
    • decreased immunoglobulin level (MGI Ref ID J:107431)
      • knockouts have lower levels of IgE and IgG2 than wild-type mice
    • decreased splenocyte proliferation (MGI Ref ID J:107431)
      • splenocytes from null mice have a lower proliferative response to secondary exposure to OVA than do wild-type
    • lung inflammation (MGI Ref ID J:107431)
      • knockout mice immunized 2x with OVA have much fewer cellular infiltrates and very little goblet cell hyperplasia in the bronchi when challenged with OVA compared wild-type controls challenged with OVA
  • respiratory system phenotype
    • lung inflammation (MGI Ref ID J:107431)
      • knockout mice immunized 2x with OVA have much fewer cellular infiltrates and very little goblet cell hyperplasia in the bronchi when challenged with OVA compared wild-type controls challenged with OVA
  • hematopoietic system phenotype
    • abnormal T cell proliferation (MGI Ref ID J:107431)
      • CD4+ T cells isolated from OVA-immunized mice show less proliferation in response to secondary antigen exposure than wild-type T cells
    • decreased splenocyte proliferation (MGI Ref ID J:107431)
      • splenocytes from null mice have a lower proliferative response to secondary exposure to OVA than do wild-type

Strain of Origin: 129

Strain genetic background: C57BL/6J

Strain Development: No obvious phenotype development. Normal Homozygous breeding

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Dermatology
  • Immunology and Inflammation
  • Models of Human Disease

Strain Origin

Donor: Warren Leonard, M.D., NIH, NHLBI

Primary Reference:

  • Al-Shami A, Spolski R, Kelly J, Fry T, Schwartzberg PL, Pandey A, Mackall CL, Leonard WJ. A role for thymic stromal lymphopoietin in CD4(+) T cell development. J Exp Med. 2004 Jul 19;200(2):159-68. PMID: 15263024 (Medline PMID: 15263024)
  • Al-Shami A, Spolski R, Kelly J, Keane-Myers A, Leonard WJ. A role for TSLP in the development of inflammation in an asthma model. J Exp Med. 2005 Sep 19;202(6):829-39. PMID: 16172260 (Medline PMID: 16172260)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
015980-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/16/2014