Strain Detail Sheet

Strain Name    :

B6.129-Nasptm1Mgor/Mmnc

Stock Number :

016128-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Nasptm1Mgor
Name: targeted mutation 1, Michael G O'Rand
Alteration at locus: Knockout
Gene Symbol: Nasp
Name: nuclear autoantigenic sperm protein (histone-binding)
Chromosome: 4
Alteration at locus: Knockout

Genetic Alterations:
A neo cassette was targeted to knockout exons 6-11.

Genotype Determination:

  • The genotyping protocol for this strain is currently not available. The MMRRC will develop a protocol if this strain is ordered.

ES Cell Line: E14 derived from 129P2/OlaHsd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Homozygous null NASP embryos do not survive to implantation but die at or before the blastocyst stage.

Heterozygous phenotype: The mice seem normal.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Nasptm1Mgor/Nasptm1Mgor
        involves: 129S/SvEv * C57BL/6
  • mortality/aging
    • complete embryonic lethality before implantation (MGI Ref ID J:116454)
      • embryos are present at E3.5 but not at E5.5 or later
  • embryogenesis phenotype
    • decreased embryo size (MGI Ref ID J:116454)
      • at E3.5, blastocyts are smaller and contain fewer cells
    • embryonic growth arrest (MGI Ref ID J:116454)
      • blastocysts collected at E3.5 fail to develop in culture
  • growth/size phenotype
    • decreased embryo size (MGI Ref ID J:116454)
      • at E3.5, blastocyts are smaller and contain fewer cells

Strain of Origin: 129S6/SvEvTac

Strain genetic background: C57BL/6

Strain Development: The 129SV/EV targeted cells were injected into blastocysts from C57BL/6 mice and the agouti offspring were backcrossed 8 times into C57BL/6.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Reproduction

Strain Origin

Donor: Michael G. O'Rand, Ph.D., University of North Carolina, Chapel Hill

Primary Reference:

Richard T. Richardson, Oleg M. Alekseev, Gail Grossman, Esther E. Widgren, Randy Thresher, Eric J. Wagner, Kelly D. Sullivan, William F. Marzluff, and Michael G. O'Rand Nuclear Autoantigenic Sperm Protein (NASP), a Linker Histone Chaperone That Is Required for Cell Proliferation J. Biol. Chem. 2006 281: 21526-21534. (Medline PMID: 16728391)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
016128-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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