Strain Name:
FVB.129S2(Cg)-Ets2tm1Rgo/Mmmh
Stock Number:
016130-MU
Citation ID:
RRID:MMRRC_016130-MU

Strain Information

Ets2tm1Rgo
Name: E26 avian leukemia oncogene 2, 3' domain; targeted mutation 1, Robert G Oshima
Synonyms: ets2db1, Ets2db
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
Ets2
Name: E26 avian leukemia oncogene 2, 3' domain
Synonyms: Ets-2
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Knockout
NCBI: 23872
HGNC: HGNC:3489
Homologene: 3838
Genetic Alterations
Replacement of DNA binding domain exons with pMC1NeopA gene.
Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype
Homozygous: Embryonic lethal D8.5. Placental insufficiency. Rescued by tetraploid embryo aggregation. Rescued adult mice display curly hair and whiskers. Extraembryonic ectoderm fails to signal to embryo for embryonic axis formation.

Heterozygous: None apparent. Delays MMTV-PyMT tumor appearance.
Mammalian Phenotype Terms
Allelic Composition: Ets2tm1Rgo/Ets2tm1Rgo (Genetic Background: either: (involves: 129S2/SvPas * 129/Sv) or (involves: 129S2/SvPas * Black Swiss) )

Allelic Composition: Ets2tm2Rgo/Ets2tm1Rgo (Genetic Background: involves: FVB/N )

MeSH Terms
  • Animals
  • Base Sequence
  • Binding Sites/genetics
  • Chimera
  • DNA Primers/genetics
  • DNA-Binding Proteins
  • Embryonic and Fetal Development/genetics
  • Embryonic and Fetal Development/physiology
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Hair/abnormalities
  • Male
  • Metalloendopeptidases/genetics
  • Mice
  • Mice, Knockout
  • Mutation
  • Phenotype
  • Polyploidy
  • Proto-Oncogene Protein c-ets-2
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/physiology
  • RNA/genetics
  • RNA/metabolism
  • Receptor, Epidermal Growth Factor/genetics
  • Receptor, Epidermal Growth Factor/physiology
  • Repressor Proteins
  • Signal Transduction
  • Trans-Activators/genetics
  • Trans-Activators/physiology
  • Transcription Factors
  • Trophoblasts/physiology
Strain Development
Targeted ES cells of 129 background were injected to C57BL/6 embryos. Chimera was bred to SwissBlack and to 129. Progeny of 129 breeding were bred to 129 background and to FVB/N 129 strain was discontinued.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Dermatology
  • Developmental Biology
  • Immunology and Inflammation
Donor
Robert Oshima, Ph.D., The Burnham Institute for Medical Research
Primary Reference
Yamamoto H, Flannery ML, Kupriyanov S, Pearce J, McKercher SR, Henkel GW, Maki RA, Werb Z, Oshima RG. Defective trophoblast function in mice with a targeted mutation of Ets2. Genes Dev. 1998 May 1;12(9):1315-26. (Medline PMID: 9573048)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
016130-MU-RESUS Litter recovered from cryo-archive $2,697.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.