Strain Detail Sheet

Strain Name    :

C57BL/6J-Itgb2m1Btlr/Mmucd

Stock Number :

016138-UCD

Other Names   :

Joker, C57BL/6-Itgb2m1Btlr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Itgb2M1Btlr
Name: mutation 1, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Itgb2
Name: integrin beta 2
Chromosome: 10
Alteration at locus: Chemically Induced

Genetic Alterations:
Sequencing at the genomic level disclosed a A to T transversion at position 7990 downstream from the distal end of exon 5. Bypass of the normal acceptor site. Confirmation of the missing exon 6 at the cDNA level.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Mononuclear cells (leukocytes, monocytes) of Joker mutants show an abolished expression of Mac-1 (CD11a-CD18), LFA-1 (CD11a-CD18) and CD11c-CD18 complex, as well as CD18 at their surface, leading to a reduction in leukocyte migration into tissues.

Heterozygous phenotype: Mononuclear cells (leukocytes, monocytes) of Joker heterozygotes show an intermediate expression (higher than Joker homozygotes, lower than wild type mice) of Mac-1, LFA-1 and CD11c-CD18 complex, as well as CD18 at their surface.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Itgb2m1Btlr/Itgb2m1Btlr
        C57BL/6J-Itgb2<m1Btlr>
  • immune system phenotype
    • immune system phenotype (MGI Ref ID J:139559)
      • mice homozygous for this mutation are not susceptible to infection by mouse cytomegalovirus (MCMV)
      • abnormal NK cell differentiation (MGI Ref ID J:139559)
        • NK cells of homozygous mutant mice arrest at the CD27+CD11b- stage
        • expression of KLRG-1 and CD43, two markers of the late stage in NK cell maturation, is reduced by half in homozygous mutant NK cells
        • despite apparent defects in NK cell maturation, NK cell numbers are normal
        • degranulation by mutant NK cells is normal
      • decreased interferon-gamma secretion (MGI Ref ID J:139559)
        • homozygous mutant NK cells fail to produce interferon (IFN) gamma upon stimulation of Ly49D, an NK cell activating receptor
        • however, IFN gamma production is normal in response to stimulation with YAC-1 or CHO-A7 cells, PMA/ionomycin, IL-12/IL-18, and antibodies to NK1.1
  • hematopoietic system phenotype
    • abnormal NK cell differentiation (MGI Ref ID J:139559)
      • NK cells of homozygous mutant mice arrest at the CD27+CD11b- stage
      • expression of KLRG-1 and CD43, two markers of the late stage in NK cell maturation, is reduced by half in homozygous mutant NK cells
      • despite apparent defects in NK cell maturation, NK cell numbers are normal
      • degranulation by mutant NK cells is normal

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: G0 C57BL/6 males were treated with ENU and crossed to C57BL/6 females. G1 males were crossed to C57BL/6 females, and females from the progeny were backcrossed to G1 males. Joker arose in the G3 generation.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cell Biology
  • Immunology and Inflammation
  • Models for Human Disease

Strain Origin

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Crozat K; Eidenschenk C; Jaeger BN; Krebs P; Guia S; Beutler B; Vivier E; Ugolini S, Impact of {beta}2 integrin deficiency on mouse natural killer cell development and function., Blood 2011 Mar 10;117(10):2874-82 (Medline PMID: 21239699)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
016138-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
016138-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
016138-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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