Strain Name:
B6;129S- Myh10tm3Rsad/Mmmh
Stock Number:
016142-MU
Citation ID:
RRID:MMRRC_016142-MU

Strain Information

Myh10tm1Rsad
Name: myosin, heavy polypeptide 10, non-muscle; targeted mutation 1, Robert S Adelstein
Synonyms: BdeltaI
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knockout
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: nonmuscle myosin heavy chain IIB, SMemb, NMHC-B, nonmuscle myosin heavy chain II-B, Myhn-2, 5730504C04Rik, myosin IIB, Fltn, Myhn2, Fltn, NMHC II-B, Myosin IIB, 9330167F11Rik
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knockout
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Genetic Alterations
Introduction of a point mutation into the nonmuscle myosin II-B heavy chain, R709C. Neomycin cassette was introduced into an intron and renders these mice hypomorphic for the mutant allele.
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Phenotype
Homozygous: R709CN mice die 15- 20 days after birth with hydocephalus and cerebellar defects which lead to abnormal gait and the inability to right themselves from a supine position. Cardiac myocytes are binucleated. Homozygotes express 20% of the expected amount of mutant myosin and unlike the R709C mutant mice can be used to study brain development. The R709C mice die during embryonic development.

Heterozygous:
Normal
Mammalian Phenotype Terms
Allelic Composition: Myh10tm1Rsad/Myh10tm1Rsad (Genetic Background: involves: 129S4/SvJae )

Allelic Composition: Myh10tm1Rsad/Myh10tm2Rsad (Genetic Background: involves: 129S4/SvJae )

MeSH Terms
  • Animals
  • Bromodeoxyuridine/metabolism
  • Cell Division/genetics
  • Cell Nucleus/metabolism
  • Cell Nucleus/pathology
  • Cell Size/genetics
  • Cells, Cultured
  • Cyclin D2
  • Cyclin D3
  • Cyclins/metabolism
  • Desmin/metabolism
  • Genotype
  • Heart Ventricles/embryology
  • Heart Ventricles/metabolism
  • Immunoblotting
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation
  • Myocardium/metabolism
  • Myocardium/pathology
  • Myocytes, Cardiac/metabolism
  • Myocytes, Cardiac/pathology
  • Myosin Heavy Chains/genetics
  • Nonmuscle Myosin Type IIB
  • Cell Movement
  • Cerebellum/abnormalities
  • Cerebellum/cytology
  • Cerebellum/metabolism
  • Facial Nerve/abnormalities
  • Facial Nerve/cytology
  • Facial Nerve/metabolism
  • Molecular Motor Proteins/chemistry
  • Molecular Motor Proteins/genetics
  • Molecular Motor Proteins/metabolism
  • Myosin Heavy Chains/chemistry
  • Myosin Heavy Chains/metabolism
  • Neurons/cytology
  • Nonmuscle Myosin Type IIB/chemistry
  • Nonmuscle Myosin Type IIB/genetics
  • Nonmuscle Myosin Type IIB/metabolism
  • Point Mutation/genetics
  • Protein Structure, Tertiary
Strain Development
129 embryonic stem cells injected into C57BL/6 blastocysts for chimera. Chimeras crossed to C57BL/6 to generate heterozygotes.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools
Donor
Robert A. Adelstein. M.D., National Institutes of Health, National Heart, Lung, and Blood Institute (NHLBI)
Primary Reference
  • Takeda K, Kishi H, Ma X, Yu ZX, Adelstein RS. Ablation and mutation of nonmuscle myosin heavy chain II-B results in a defect in cardiac myocyte cytokinesis. Circ Res. 2003 Aug 22;93(4):330-7. Epub 2003 Jul 31. (Medline PMID: 12893741)
  • Ma X, Kawamoto S, Hara Y, Adelstein RS. A point mutation in the motor domain of nonmuscle myosin II-B impairs migration of distinct groups of neurons. Mol Biol Cell. 2004 Jun;15(6):2568-79. Epub 2004 Mar 19. (Medline PMID: 15034141)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
016142-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
016142-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.