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Strain Detail Sheet

Strain Name:
B6.Cg-Mitfmi-rw/Mitfmi-sp/Mmmh
Stock Number:
000163-MU
Citation ID:
RRID:MMRRC_000163-MU
Other Names:
microphthalmia transcription factor
Major Collection:
Mammalian Pigmentation

Strain Information

Gene Details

Mitfmi-sp
Name: melanogenesis associated transcription factor; microphthalmia spotted
Synonyms: MITFsp, sp
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Mitfmi-rw
Name: melanogenesis associated transcription factor; red eyed white
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Mitf
Name: melanogenesis associated transcription factor
Synonyms: mi, wh, BCC2, bHLHe32, Gsfbcc2
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Spontaneous Mutation
NCBI: 17342
HGNC: HGNC:7105
Homologene: 4892

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Spontaneous mutation with a large genomic deletion approximately 6 kb upstream of the melanocyte-specific 5' end.
Genotype Determination

Strain Description

Phenotype
Description of White Spotting mutants

Mitfrw / mitfrw mice are white, usually with black spots on or about the area of eyes and ears (on a B6 background, and in other locations on other backgrounds). Homozygous mice have small red eyes. Mitfrw / mitfsp mice are black with a white belly spot and may have a white head spot and usually half the tail is white (these can be almost impossible to distinguish from the background strain C57BL/6J-Mitfmi-sp, (MMRRC:000153). Heterozygous mice have black eyes.
Mammalian Phenotype Terms
Allelic Composition: Mitfmi-sp/Mitfmi-sp (Genetic Background: B6.Cg-MitfMi-wh/Mitfmi-sp/J )
Allelic Composition: MitfMi-wh/Mitfmi-sp (Genetic Background: B6.Cg-MitfMi-wh/Mitfmi-sp/J )
Allelic Composition: MitfMi/Mitfmi-sp (Genetic Background: B6.Cg-MitfMi/Mitfmi-sp )
Allelic Composition: MitfMi/Mitfmi-sp (Genetic Background: involves: C57BL/6J )
Allelic Composition: Mitfmi-rw/Mitfmi-rw (Genetic Background: CBA/J )
MeSH Terms
  • Animals
  • DNA-Binding Proteins/genetics
  • Eye Abnormalities
  • Genetic Complementation Test
  • Heterozygote
  • Mice
  • Microphthalmia-Associated Transcription Factor
  • Mutation
  • Transcription Factors/genetics
  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • DNA-Binding Proteins/chemistry
  • Deafness/genetics
  • Disease Models, Animal
  • Eye Color/genetics
  • Gene Expression Regulation, Developmental
  • Genes, Dominant
  • Hair Color/genetics
  • Helix-Loop-Helix Motifs
  • Humans
  • Leucine Zippers
  • Mast Cells/pathology
  • Melanocytes/pathology
  • Mice, Inbred Strains
  • Mice, Mutant Strains
  • Microphthalmos/genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Neural Crest/pathology
  • Osteopetrosis/genetics
  • Phenotype
  • Protein Conformation
  • RNA Splicing
  • Tooth Abnormalities/genetics
  • Transcription Factors
  • Waardenburg Syndrome/classification
  • Waardenburg Syndrome/genetics
Strain Development
Spontaneous mutation in CBA/J mouse colony. Backcrossed onto the C57BL/6J-Mitfmi-sp strain. Maintained heterozygous with the mi-sp allele by mating a homozygous males to C57BL/6J-Mitfmi-sp females.
Suggested Control Mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Pigmentation
  • Developmental Biology
  • Gene Regulations
  • Signaling molecules
  • Pattern formation
  • Cancer incidence
  • Obesity
  • Diabetes

Strain Origin

Donor
Lynn Lamoreux, Ph.D., Texas Agricultural and Mechanical University.
Primary Reference
  • Steingrimsson E, Arnheiter H, Hallsson JH, Lamoreux ML, Copeland NG, Jenkins NA. Interallelic complementation at the mouse Mitf locus. Genetics. 2003 Jan;163(1):267-76. (Medline PMID: 12586714)
  • Steingrimsson E, Moore KJ, Lamoreux ML, Ferre-D'Amare AR, Burley SK, Zimring DC, Skow LC, Hodgkinson CA, Arnheiter H, Copeland NG. Molecular basis of mouse microphthalmia (mi) mutations helps explain their developmental and phenotypic consequences. Nat Genet. 1994 Nov;8(3):256-63. (Medline PMID: 7874168)
  • Southard JL, Red-eyed white (mi-rw), Mouse News Letter 1974, 51:23. (Medline: Not available)

Colony and Husbandry Information

Special Considerations

Feed 9% fat breeder chow.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
See Phenotype description

Breeding

MMRRC Breeding System
Backcross
Breeding Scheme(s)
C57BL/6J-Mitfmi-sp female x Homozygous male
Generation
N10+
Overall Breeding Performance
Good

Reproductive Statistics

Average litter size
6
Recommended wean age
4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
000163-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit		 Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000163-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
000163-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.