Strain Name    : B6.Cg-Mitfmi-bw/Mitfmi-sp/Mmmh

Stock Number : 000165-MU

Gene Information

(provided by MGI)
Allele Symbol: Mitfmi-bw
Name: black eyed white
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Allele Symbol: Mitfmi-sp
Name: microphthalmia spotted
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Gene Symbol: Mitf
Name: microphthalmia-associated transcription factor
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Genetic Alterations:
In this allele, a 7.2 kb novel L1 element was inserted into intron 3. This insertion reduces the amount of mRNAs for two splicing isoforms of this gene and abolishes expression of a third isoform.

Genotype Determination:

ES Cell Line:

Phenotype

Description of White Spotting mutants

Homozygous mice are white. Heterozygous mice are largely black with a fairly large white belly spot. Wildtype mice are black and if they have a white belly spot, it is very small. Black mice without the large belly spot do not carry the mutant allele of interest. In addition to pigmentation defects, mice mutant at the target locus are known to have defects of the eyes, mast cells, reproductive system, peristalsis, osteoclasts (giving rise to absence of teeth), and the inner ear. This mutant allele has not been studied with regard to these defects, but the eyes and teeth appear normal. Though no pigmentation is observed in the skin and hair of the homozygote, pigment cells have been found in the skin of newborns.

Mammalian Phenotype Terms:
Allelic Composition: Mitfmi-bw/Mitfmi-bw (Genetic Background: C3H )

Allelic Composition: Mitfmi-sp/Mitfmi-sp (Genetic Background: B6.Cg-MitfMi-wh/Mitfmi-sp/J )

Allelic Composition: MitfMi-wh/Mitfmi-sp (Genetic Background: B6.Cg-MitfMi-wh/Mitfmi-sp/J )

Allelic Composition: MitfMi/Mitfmi-sp (Genetic Background: B6.Cg-MitfMi/Mitfmi-sp )

Allelic Composition: MitfMi/Mitfmi-sp (Genetic Background: involves: C57BL/6J )

Strain of Origin: C3H

Strain genetic background: C57BL/6-Mitfmi-sp (MMRRC:000153)

Strain Development: Heterozygous mice are backcrossed to C57BL/6-Mitfmi-sp every generation. Heterozygotes are sib mated to produce homozygotes when needed.

Suggested Control Mice:

  • Pigmentation
  • Eye development
  • Inner ear development

Donor: Dr. M. L. Lamoreux, Texas A&M University

Primary Reference:

  • Steingrimsson E; Moore KJ; Lamoreux ML; Ferre-D'Amare AR; Burley SK; Zimring DC; Skow LC; Hodgkinson CA; Arnheiter H; Copeland NG; Jenkins NA, Molecular basis of mouse microphthalmia (mi) mutations helps explain their developmental and phenotypic consequences [see comments], Nat Genet 1994 Nov;8(3):256-63. (Medline: 7874168)
  • Hallsson JH, Favor J, Hodgkinson C, Glaser T, Lamoreux ML, Magnusdottir R, Gunnarsson GJ, Sweet HO, Copeland NG, Jenkins NA, Steingrimsson E. Genomic, transcriptional and mutational analysis of the mouse microphthalmia locus. Genetics. 2000 May;155(1):291-300. (Medline: 10790403)
  • Steingrimsson E, Arnheiter H, Hallsson JH, Lamoreux ML, Copeland NG, Jenkins NA. Interallelic complementation at the mouse mitf locus. Genetics. 2003 Jan;163(1):267-76. (Medline: 12586714)

Colony and Husbandry Information

Diet should contain 9% fat. Homozygous mice have extremely poor breeding performance and should not be used for breeding.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Homozygous: white; Heterozygous: mostly black with large white belly spot.

Other:

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • Heterozygous Female x C57BL/6-Mitfmi-sp Male
  • Heterozygous Female x Heterozygous Male (to obtain Homozygous mice)

Generation: Unknown

Overall Breeding Performance: Good (Heterozygous Mice)

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Poor Poor
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: Unknown Unknown

Average litter size: Unknown

Recommended wean age: 4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
000165-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000165-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
000165-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.