Strain Name    : B6.Cg-Tg(H2-Kb-Il21)5Wjl/Mmnc

Stock Number : 016978-UNC

Gene Information

(provided by MGI)
Allele Symbol: Tg(H2-Kb-IL21)5Wjl
Name: transgene insertion 5, Warren J Leonard
Alteration at locus: Transgenic
Promoter: H2-K
Name: histocompatibility 2, K region
Alteration at locus: Transgenic
Transgene: Il21
Name: interleukin 21
Alteration at locus: Transgenic
Genetic Alterations:
Donor generated IL- 21 TG mice using a vector that drives expression in T, B, and NK cells. Murine or human IL-21 cDNA constructs containing V5 and His tags were generated by PCR and inserted into pHSE, a plasmid in which the expressed cDNA is under the control of the H-2kb promoter and IgM enhancer.TG mice were then generated by standard procedures

Genotype Determination:

ES Cell Line: Not Applicable

Phenotype

Homozygous phenotype: None

Hemizygous phenotype: Using IL-21-transgenic mice it has been demonstrated that although IL-21 induces death of resting B cells, it promotes differentiation of B cells into postswitch and plasma cells. Thus, IL-21 differentially influences B cell fate depending on the signaling context, IL-21 can be proapoptotic for B cells in vitro yet critical for Ag-specific Ig production in vivo.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Tg(H2-Kb-IL21)5Wjl/-
        involves: 129
  • immune system phenotype
    • abnormal B cell differentiation (MGI Ref ID J:93740)
      • B cell differentiation is driven towards postswitch cells unlike in wild-type mice
      • abnormal class switch recombination (MGI Ref ID J:93740)
        • mice exhibit an increase in Ig class-switched B cells compared to in wild-type mice
      • decreased transitional stage T2 B cell number (MGI Ref ID J:93740)
      • increased immature B cell number (MGI Ref ID J:93740)
        • in the periphery
        • increased transitional stage T1 B cell number (MGI Ref ID J:93740)
    • decreased mature B cell number (MGI Ref ID J:93740)
      • decreased follicular B cell number (MGI Ref ID J:93740)
      • decreased marginal zone B cell number (MGI Ref ID J:93740)
    • increased B cell apoptosis (MGI Ref ID J:93740)
    • increased IgG1 level (MGI Ref ID J:93740)
    • increased IgM level (MGI Ref ID J:93740)
  • hematopoietic system phenotype
    • abnormal B cell differentiation (MGI Ref ID J:93740)
      • B cell differentiation is driven towards postswitch cells unlike in wild-type mice
      • abnormal class switch recombination (MGI Ref ID J:93740)
        • mice exhibit an increase in Ig class-switched B cells compared to in wild-type mice
      • decreased transitional stage T2 B cell number (MGI Ref ID J:93740)
      • increased immature B cell number (MGI Ref ID J:93740)
        • in the periphery
        • increased transitional stage T1 B cell number (MGI Ref ID J:93740)
    • decreased mature B cell number (MGI Ref ID J:93740)
      • decreased follicular B cell number (MGI Ref ID J:93740)
      • decreased marginal zone B cell number (MGI Ref ID J:93740)




Strain of Origin: 129

Strain genetic background: C57BL/6

Strain Development: No obvious phenotype development. Normal Hemizygous breeding. TG X WT

Suggested Control Mice:

  • Wildtype littermates

  • Apoptosis
  • Immunology and Inflammation
  • Models for Human Disease

Donor: Warren Leonard, M.D., NIH

Primary Reference:

Ozaki K, Spolski R, Ettinger R, Kim HP, Wang G, Qi CF, Hwu P, Shaffer DJ, Akilesh S, Roopenian DC, Morse HC 3rd, Lipsky PE, Leonard WJ. Regulation of B cell differentiation and plasma cell generation by IL-21, a novel inducer of Blimp-1 and Bcl-6. J Immunol. 2004 Nov 1;173(9):5361-71. (Medline PMID: 15494482)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
016978-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.