Strain Detail Sheet

Strain Name    :

B6.129S4-Mc5rtm1Cone/Mmucd

Stock Number :

016980-UCD

Other Names   :

Melanocortin 5 Receptor knockout mouse (MC5-R), B6.129S4-Mc5rtm1Cone/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mc5rtm1Cone
Name: targeted mutation 1, Roger D Cone
Alteration at locus: Knockout
Gene Symbol: Mc5r
Name: melanocortin 5 receptor
Chromosome: 18
Alteration at locus: Knockout

Genetic Alterations:
A 650 bp fragment of the single coding exon of the MC5-R gene was disrupted by homologous recombination using a neomycin cassette.

Genotype Determination:

ES Cell Line: AK7 derived from 129S4/SvJaeSor

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: The MC5-R knockout mouse is characterized by defects in exocrine function. Of those described are a defect in production and stress-related synthesis of porphyrins by the harderian gland, defects in the ACTH/MSH regulated protein secretion by the lacrimal gland, and decreased production of sebacious lipids. The MC5-R is implicated in pheremone-induced aggression as the knockout mouse displays reduced pheremonal signalling for aggression in male mice acting on donors rather than their opponents.

Heterozygous phenotype: The heterozygous MC5-R mouse exhibits an intermediate phenotype relative to that of the wild-type and knockout mouse.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Mc5rtm1Cone/Mc5rtm1Cone
        B6.129S4-Mc5r<tm1Cone>
  • endocrine/exocrine gland phenotype
    • abnormal Harderian gland physiology (MGI Ref ID J:44727)
      • little or no porphyrin synthesis in response to restraint stress
  • behavior/neurological phenotype
    • decreased aggression towards males (MGI Ref ID J:101848)
      • homozygous males are less aggressive and more defensive when paired with wildtype males, however social and nonsocial behaviors are unaltered and levels of aggression and defense are unaltered when paired with gonadectomized opponents or with other homozygous males
      • homozygous males exhibit even less aggression and more defense in the dark phase when paired with wildtype males
      • aggressive behavior is not elevated in homozygous males during the dark phase as it is in wildtype


The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Mc5rtm1Cone/Mc5rtm1Cone
        involves: 129S4/SvJaeSor * 129S6/SvEvTac
  • homeostasis/metabolism phenotype
    • impaired adaptive thermogenesis (MGI Ref ID J:44727)
      • core body temperature decreases more during swimming at 32 degrees Celcius and takes a longer time to return to baseline compared to controls
      • when challenged with cold air, homozygotes undergo mild hypothermia, while controls increase core temperature and maintain the higher temperature for at least 3 hours
  • endocrine/exocrine gland phenotype
    • abnormal Harderian gland physiology (MGI Ref ID J:44727)
      • little or no porphyrin s ynthesis from Harderian gland
    • abnormal lacrimal gland physiology (MGI Ref ID J:44727)
      • do not observe melanocortin-stimulated protein secretion as in wildtype
    • abnormal sebaceous lipid secretion (MGI Ref ID J:44727)
      • reduction in sterol esters in sebum
  • vision/eye phenotype
    • abnormal lacrimal gland physiology (MGI Ref ID J:44727)
      • do not observe melanocortin-stimulated protein secretion as in wildtype
  • integument phenotype
    • abnormal coat/ hair morphology (MGI Ref ID J:44727)
      • absorb almost twice as much water as wildtype and need more time for fur to dry after swimming due to impaired water repulsion
      • decrease in hair lipid content indicated by a 15-20% reduction in acetone-extractable materials from hair
    • abnormal sebaceous lipid secretion (MGI Ref ID J:44727)
      • reduction in sterol esters in sebum

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: Positive ES cells were microinjected into blastocysts of C57BL/6J mice. The two clones were backcrossed 7-9 generations into C57BL/6J to make congenic strains.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Dermatology
  • Diabetes/Obesity
  • Metabolism
  • Reproduction

Strain Origin

Donor: Roger D. Cone, Ph.D., Vanderbilt University

Primary Reference:

  • Chen W, Kelly MA, Opitz-Araya X, Thomas RE, Low MJ, Cone RD. Exocrine gland dysfunction in MC5-R-deficient mice: evidence for coordinated regulation of exocrine gland function by melanocortin peptides. Cell. 1997 Dec 12;91(6):789-98. (Medline PMID: 9413988)
  • Morgan C, Cone RD. Melanocortin-5 receptor deficiency in mice blocks a novel pathway influencing pheromone-induced aggression. Behav Genet. 2006 Mar;36(2):291-300. Epub 2006 Jan 12. (Medline PMID: 16408249)

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
016980-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
016980-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
016980-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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