Strain Detail Sheet

Strain Name    :

STOCK Myh10tm7Rsad/Mmnc

Stock Number :

016981-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Myh10tm7Rsad
Name: targeted mutation 7, Robert S Adelstein
Alteration at locus: Targeted Mutation
Gene Symbol: Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Chromosome: 11
Alteration at locus: Targeted Mutation

Genetic Alterations:
Introduction of Neo cassette into intron near exon 2. LoxP sites flank Neo cassette and exon 2.

Genotype Determination:

ES Cell Line: CMTI-1 derived from B6;129S

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: There is no phenotype.

Heterozygous phenotype: There is no phenotype.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

Strain of Origin: B6;129

Strain genetic background: STOCK (C57BL/6, 129, FVB/N, IVR)

Strain Development: Embryonic stem cells (129) were injected into C57BL/6 blastocysts to generate chimeras. Chimeras were crossed to C57BL/6 to generate heterozygotes. The line was subsequently crossed to an alpha MHC Cre mouse (mixed background of FVB/N and ICR) before being sent to the MMRRC. The line no longer contains the Cre allele.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Donor: Robert S. Adelstein, M.D., NIH, NHLBI

Primary Reference:

Ma X, Takeda K, Singh A, Yu ZX, Zerfas P, Blount A, Liu C, Towbin JA, Schneider MD, Adelstein RS, Wei Q. Conditional ablation of nonmuscle myosin II-B delineates heart defects in adult mice. Circ Res. 2009 Nov 20;105(11):1102-9. Epub 2009 Oct 8. (Medline PMID: 19815823)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
016981-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
016981-UNC-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 04/24/2014