Strain Name:
C57BL/6J-Tirapm1Btlr/Mmucd
Stock Number:
016982-UCD
Citation ID:
RRID:MMRRC_016982-UCD
Other Names:
Torpid, C57BL/6-Tirapm1Btlr/Mmcd
Major Collection:

Strain Information

Tirapm1Btlr
Name: toll-interleukin 1 receptor (TIR) domain-containing adaptor protein; mutation 1, Bruce Beutler
Synonyms: torpid
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
Tirap
Name: toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
Synonyms: C130027E04Rik, Mal, wyatt, MyD88-adapter-like, Mal
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 117149
Homologene: 14285
Genetic Alterations
An N-ethyl-N- nitrosourea (ENU)-induced A-> T mutation in the Tirap gene, located on chromosome 9, is responsible for the Torpid phenotype. The lesion is a splicing mutation in the acceptor site of exon 3 (AG -> TG). The presence of an in-frame TGA in the un-spliced intron 2 results in a prematurely truncated protein that has six C-terminal amino acids replaced by three unrelated residues.
Genotype Determination
Phenotype
Homozygous: Torpid mice display no response to peptidoglycan, whereas responses to other TLR ligands (CpG, poly I:C, and resiquimod) is normal.

Heterozygous: Not characterized
Mammalian Phenotype Terms
Allelic Composition: Tirapm1Btlr/Tirapm1Btlr (Genetic Background: C57BL/6J-Tirapm1Btlr )

  • immune system
Strain Development
G0 C57BL/6 males are treated with ENU and bred with C57BL/6 females. G1 males are bred with C57BL/6 females, and females from the progeny are backcrossed with G1 males. Torpid arose from the G3 generation.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Immunology and Inflammation
Donor
Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference
For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
016982-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
016982-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
016982-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.