Strain Name:
B6;129P2-Mmp20tm1Jdb/Mmmh
Stock Number:
016999-MU
Citation ID:
RRID:MMRRC_016999-MU

Strain Information

Mmp20tm1Jdb
Name: matrix metallopeptidase 20 (enamelysin); targeted mutation 1, John D Bartlett
Synonyms: enamelysin-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Mmp20
Name: matrix metallopeptidase 20 (enamelysin)
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knockout
NCBI: 30800
VEGA: 9
HGNC: HGNC:7167
Homologene: 21001
Genetic Alterations
Majority of intron 4 and exon 5 of murine Mmp-20 was replaced by a phosphoglycerate kinase promotor-controlled hypoxanthine-guanine phosphoribosyl transferase (Hprt) minigene.
Genotype Determination
ES Cell Line
HM-1 derived from 129P2/OlaHsd-Hprtb-m3
Phenotype
Homozygous: Faulty tooth enamel

Heterozygous: Same as wild-type
Mammalian Phenotype Terms
Allelic Composition: Mmp20tm1Jdb/Mmp20tm1Jdb (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

MeSH Terms
  • Amelogenesis Imperfecta/genetics
  • Amelogenin
  • Animals
  • Dental Enamel Proteins/genetics
  • Disease Models, Animal
  • Electrophoresis, Polyacrylamide Gel
  • Exons
  • Genetic Vectors
  • Genotype
  • Heterozygote
  • Introns
  • Matrix Metalloproteinase 20
  • Matrix Metalloproteinases/genetics
  • Matrix Metalloproteinases/physiology
  • Mice
  • Mice, Transgenic
  • Microscopy, Electron, Scanning
  • Models, Genetic
  • Molar/physiology
  • Molar/ultrastructure
  • Mutation
  • Peptides/chemistry
  • Phenotype
  • RNA/metabolism
  • Amelogenesis/genetics
  • Dental Enamel/chemistry
  • Dental Enamel Proteins/analysis
  • Hardness
  • Kallikreins/genetics
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Minerals/analysis
  • Phosphates/analysis
  • Spectroscopy, Fourier Transform Infrared
  • Alternative Splicing/genetics
  • Alternative Splicing/physiology
  • Amelogenin/genetics
  • Amelogenin/metabolism
  • Base Sequence
  • Dental Enamel/enzymology
  • Dental Enamel/metabolism
  • Exons/physiology
  • Gene Expression Regulation, Developmental/physiology
  • Intracellular Signaling Peptides and Proteins/genetics
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Matrix Metalloproteinase 20/genetics
  • Matrix Metalloproteinase 20/metabolism
  • Molar/enzymology
  • Molar/metabolism
  • Molecular Sequence Data
  • Protein Isoforms
  • RNA, Messenger/metabolism
  • Signal Transduction/genetics
Strain Development
Mutant mice were mated to C57BL/6 to generate heterozygous animals for the targeted gene. These were subsequently interbred to generate homozygous mutant progeny. Donor performed sib mating to reduce background effects.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Research Tools
Donor
John D Bartlett, Ph.D., The Forsyth Institute
Primary Reference

Caterina JJ, Skobe Z, Shi J, Ding Y, Simmer JP, Birkedal-Hansen H, Bartlett JD. Enamelysin (matrix metalloproteinase 20)-deficient mice display an amelogenesis imperfecta phenotype. J Biol Chem. 2002 Dec 20;277(51):49598-604. Epub 2002 Oct 21. (Medline PMID: 12393861)

Bartlett JD, Beniash E, Lee DH, Smith CE. Decreased mineral content in MMP-20 null mouse enamel is prominent during the maturation stage. J Dent Res. 2004 Dec;83(12):909-13. (Medline PMID: 15557396)

Bartlett JD, Ball RL, Kawai T, Tye CE, Tsuchiya M, Simmer JP. Origin, splicing, and expression of rodent amelogenin exon 8. J Dent Res. 2006 Oct;85(10):894-9. (Medline PMID: 16998127)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
016999-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
016999-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.