Strain Name:
FVB-Tg(tetO-Jun)5Nes/Mmmh
Stock Number:
017221-MU
Citation ID:
RRID:MMRRC_017221-MU

Strain Information

tetOp
Name: tetracycline operon
Type: Gene
Species: Synthetic
Chromosome:
Jun
Name: jun proto-oncogene
Synonyms: Junc, c-jun
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16476
HGNC: HGNC:6204
Homologene: 1679
Tg(tetO-Jun)5Nes
Name: transgene insertion 5, Eric Nestler
Synonyms: TetOp-FLAG-deltac-Jun, TetOp-FLAG-deltacJun line E, E line
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Clone #63026 comprising a 2.6kb insert encoding mouse c-Jun plus FLAG tag plus pTetOpHGHpA to generate pTetOp-FLAG-?c-Jun
Genotype Determination
Phenotype
Homozygous: Not characterized 

Hemizygous: No apparent phenotype
MeSH Terms
  • Animals
  • Behavior, Addictive/genetics
  • Behavior, Addictive/metabolism
  • Brain/metabolism
  • Cocaine/pharmacology
  • Gene Expression Regulation/physiology
  • Genes, Dominant/physiology
  • Humans
  • Male
  • Mice
  • Mice, Transgenic
  • Motor Activity/drug effects
  • Motor Activity/physiology
  • Mutation/physiology
  • PC12 Cells
  • Proto-Oncogene Proteins c-jun/biosynthesis
  • Proto-Oncogene Proteins c-jun/genetics
  • Rats
Strain Development
Original founder (E) mated to FVB/NJ female. Subsequent generations mated to FVB/NJ mice, alternating males and females to not sex link gene. Delivered to MMRRC on N11.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Neurobiology
  • Research Tools
  • Drug Addiction
Donor
Eric Nestler, M.D., Ph.D., University of Texas Southwestern Medical School
Primary Reference
Peakman MC, Colby C, Perrotti LI, Tekumalla P, Carle T, Ulery P, Chao J, Duman C, Steffen C, Monteggia L, Allen MR, Stock JL, Duman RS, McNeish JD, Barrot M, Self DW, Nestler EJ, Schaeffer E. Inducible, brain region-specific expression of a dominant negative mutant of c-Jun in transgenic mice decreases sensitivity to cocaine. Brain Res. 2003 Apr 25;970(1-2):73-86. (Medline PMID: 12706249)

Colony and Husbandry Information

No special requirements - Donor reports the mice like Nestlets or other nesting material.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
017221-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
017221-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
017221-MU-RESUS Litter recovered from cryo-archive $2,028.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.