Strain Name:
STOCK Mib1tm2Art/Mmnc
Stock Number:
017224-UNC
Citation ID:
RRID:MMRRC_017224-UNC

Strain Information

Mib1tm2Art
Name: MIB E3 ubiquitin protein ligase 1; targeted mutation 2, Karen Artzt
Synonyms: mib1-cko
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Conditional
Mib1
Name: MIB E3 ubiquitin protein ligase 1
Synonyms: E430019M12Rik, mindbomb, Mib, mind bomb-1, skeletrophin
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Conditional
NCBI: 225164
Homologene: 10810
Genetic Alterations
Neo-cassette was inserted in intron 2 along with loxP sites flanking exon 2 and neo- cassette. Heterzygotes were then mated to general deleter strain EIIa- Cre to remove neo-cassette to generate exon 2.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype

    Homozygous: viable, fertile and healthy

    Heterozygous: viable, fertile and healthy

    Mammalian Phenotype Terms
    Allelic Composition: Mib1tm2Art/Mib1tm2Art (Genetic Background: involves: 129S6/SvEvTac )

    • phenotype not analyzed
    Strain Development
    Original targeted mutation was in 129S6. The chimera was mated to BTNTTF to generate heterozygotes. These heterozygous mice were then mated to the EIIa-Cre strain (C57BL/6NCr) and removed neo-cassette to generate the mib1-cko mice. These mib1-cko mice have been maintained as sib-mated.
    Suggested Control Mice

    Wild-type littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Apoptosis
    • Cardiovascular
    • Cell Biology
    • Developmental Biology
    • Neurobiology
    Donor
    Karen Artzt, Ph.D., Ashbel Smith Professor Emeritus, The University of Texas, Austin
    Primary Reference
    Please note: This strain is unpublished and there are no plans to publish.

    Colony and Husbandry Information

    Donor fed mouse chow with high lipid content for nursing mothers -- usually mothers were not removed from parental cage but raised one litter in same cage as all adults with males and litter survived and thrived.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    017224-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    017224-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.