Strain Name:
BTNTTF.129S6-Mib2tm1Art/Mmnc
Stock Number:
017226-UNC
Citation ID:
RRID:MMRRC_017226-UNC
Other Names:
Zzank1

Strain Information

Mib2tm1Art
Name: mindbomb E3 ubiquitin protein ligase 2; targeted mutation 1, Karen Artzt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Mib2
Name: mindbomb E3 ubiquitin protein ligase 2
Synonyms: Zzank1, 2210008I11Rik
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knockout
NCBI: 76580
Homologene: 16062
Genetic Alterations
Neo-cassette and B-galactosidase were engineered to remove first eleven exons.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype
    Homozygous: Viable, BT, tufted non-distinguishable from wild type littermates or heterozygotes

    Heterozygous:
    Viable, BT, tufted non-distinguishable from wild type littermates or homozygotes
    Mammalian Phenotype Terms
    Allelic Composition: Mib2tm1Art/Mib2tm1Art (Genetic Background: BTNTTFArt.129S6-Mib2tm1Art )

    MeSH Terms
    • Animals
    • Embryo, Mammalian/embryology
    • Embryo, Mammalian/metabolism
    • Gene Deletion
    • Gene Expression Regulation
    • Gene Targeting
    • Mice
    • Neural Tube Defects/embryology
    • Neural Tube Defects/genetics
    • Neural Tube Defects/pathology
    • Penetrance
    • Ubiquitin-Protein Ligases/deficiency
    • Ubiquitin-Protein Ligases/genetics
    • Ubiquitin-Protein Ligases/metabolism
    Strain Development
    Male chimera of 129S6 and C57BL/6 were mated to BTNTTf females to obtain heterozygotes (genotyped). At the time of donation to the MMRRC, the mice were in backcross 7 to BTNTf.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Apoptosis
    • Cell Biology
    • Developmental Biology
    • Neurobiology
    Donor
    Karen Artzt, Ph.D. and Ashbel Smith, Ph.D., The University of Texas at Austin
    Primary Reference

    Wu JI, Rajendra R, Barsi JC, Durfee L, Benito E, Gao G, Kuruvilla M, Hrdlickova R, Liss AS, Artzt K. Targeted disruption of Mib2 causes exencephaly with a variable penetrance. Genesis. 2007 Nov;45(11):722-7. (Medline PMID: 17987667)


    Colony and Husbandry Information

    Donor fed mouse chow with high liipid content for nursing mothers. Donor notes: usually mothers are not removed from parental cage but raise one litter in same cage as all adults with males and litter survives and thrives.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    017226-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    017226-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    017226-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.