Strain Detail Sheet

Strain Name    :

BTNTTF.Cg-T2Tg(H2K)1Art/Mmnc

Stock Number :

017227-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: T2Tg(H2K)1Art
Name: transgene insertion 1, Karen Artzt
Alteration at locus: Transgenic (random, gene disruption)
Gene Symbol: T2
Name: brachyury 2
Alteration at locus: Transgenic (random, gene disruption)

Genetic Alterations:

Genotype Determination:

  • The genotyping protocol for this strain is currently not available. The MMRRC will develop a protocol if this strain is ordered.

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: lethal

Heterozygous phenotype: short-tailed no more than 50%


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
T2Tg(H2K)1Art/T2+
        involves: B10.M * BTNTTF/nev * CBA
  • limbs/digits/tail phenotype
    • kinked tail (MGI Ref ID J:29666)
      • occasional kink at end of tail
    • short tail (MGI Ref ID J:29666)
      • tail length is 90% of normal, varying in a narrow range
      • incomplete penetrance in 7% of carriers from heterozygote by wild-type matings
T2Tg(H2K)1Art/T2Tg(H2K)1Art
        involves: B10.M * BTNTTF/nev * CBA
  • mortality/aging
    • complete embryonic lethality during organogenesis (MGI Ref ID J:29666)
      • at E11-11.5 most of the abnormal embryos are moribund
      • although there is an allantoic connection, death is shortly after E11.5 when development ceases
  • embryogenesis phenotype
    • embryonic growth retardation (MGI Ref ID J:29666)
  • growth/size phenotype
    • embryonic growth retardation (MGI Ref ID J:29666)
  • cardiovascular system phenotype
    • abnormal heart tube morphology (MGI Ref ID J:29666)
      • the heart tube leaves the body cavity and makes two right angles before returning to the body cavi ty
      • cardia bifida (MGI Ref ID J:29666)
        • hearts of 10-15% of developing embryos unfolded and functional
  • limbs/digits/tail phenotype
    • absent tail (MGI Ref ID J:29666)
      • E10.5 embryos are tailless

Strain of Origin: B10.M

Strain genetic background: BAgNTTf [BTBRTF/art and this stands for black and tan (a), Brachy (original T deletion) and tufted (tf). This strain segregates for T and when no T is involved donor calls it BTNTTF.]

Strain Development: Obtained original mice from Larry Pease at Mayo Clinic in 1992 -- mice were missing hind limbs which later was determined to be behavioral by the females but the short-tail was interesting and resembled the classic Brachyury.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Models for Human Disease

Strain Origin

Donor: Karen Artzt, Ph.D., University of Texas at Austin

Primary Reference:

  • Rennebeck GM, Lader E, Chen Q, Bohm RA, Cai ZS, Faust C, Magnuson T, Pease LR, Artzt K. Is there a Brachyury the Second? Analysis of a transgenic mutation involved in notochord maintenance in mice. Dev Biol. 1995 Nov;172(1):206-17. (Medline PMID: 7589801)
  • Rennebeck G, Lader E, Fujimoto A, Lei EP, Artzt K. Mouse Brachyury the Second (T2) is a gene next to classical T and a candidate gene for tct. Genetics. 1998 Nov;150(3):1125-31. (Medline PMID: 9799264)

Colony and Husbandry Information

Special Considerations

Fed mouse chow with high lipid content for nursing mothers -- usually mothers are not removed from parental cage but raise one litter in same cage as all adults with males and litter survives and thrives.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
017227-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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