Strain Detail Sheet

Strain Name:
C57BL/6J-KitW-2J/cLmMmmh
Stock Number:
000175-MU
Citation ID:
RRID:MMRRC_000175-MU
Major Collection:
Mammalian Pigmentation

Strain Information

Gene Details

KitW-2J
Name: KIT proto-oncogene receptor tyrosine kinase; dominant spotting 2 Jackson
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Spontaneous Mutation
Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: c-KIT, belly-spot, CD117, SCO5, Tr-kit, SOW3, Steel Factor Receptor, Gsfsco1, Gsfsow3, Dominant white spotting, Gsfsco5, SCO1
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Spontaneous Mutation
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
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Strain Description

Phenotype
Description of White Spotting mutants
Description of White Kit mutants

A dominant mutation that is lethal in the homozygous state. Mice that carry the mutant allele are black with white spotting; homozygous +/+ mice are solid black. Heterozygous mice with the mutant allele have fewer pigment cells (more white areas) than heterozygous mutant mice on the C57BL/J background.

MMRRC:000173 was selected for normal expression level of the KitW-2J mutation compared to MMRRC:000174 in which hi level expression was selected for and MMRRC:000175 in which low level expression of the mutation was selected for by continued inbreeding.

In this collection of mice, the identical allelic origin is expressed in five different degrees of severity (stocks 173, 174, 175, 177, 178), depending upon the congenic background, and within the congenic mice upon two (or more) discrete modifying genes that have been maintained by selection. This stock (MMRRC:000175) is maintained by selection and has the least amount of white, sometimes only a belly spot. The orginal mutation occurred in the C57 BL/6J stocks of Dr. Elizabeth Russell. The allele is homozygous lethal. The original stock (MMRRC:000173) has been maintained with selection for higher degrees of spotting. Therefore it is interesting that mice occasionally occur with approximately half as much white, compared with the norm. Backcrossing to C57BL/6J increases incidence of these events. These low-spot mice breed true without reversion to the parental phenotype, and have been selected and maintained as (MMRRC:000175). The allele from the original stock, (MMRRC:000173), was also backcrossed onto a different inbred strain, JU/CtLm where the spotted mice have at least twice the extent of white spotting (MMRRC:000177). This stock does not periodically throw offspring with reduced levels of spotting, but did give rise to one offspring with a yet greater degree of spotting that is inherited as a semi-dominant trait. Mice that are heterozygous for the KitW-2J allele and homozygous for the hi-spot trait on the JU/CtLm background are almost completely white (MMRRC:000178), whereas mice of the C57BL/6J-W-2J/+ lo-spot stock (MMRRC:000175) are often almost completely lacking defined white spots. The hi-spot trait was then crossed back onto C57BL/6J, with strong selection in the presence of KitW-2J, resulting in yet another phenotype consisting of considerably more white than is normal for this allele on a C57BL/6J background. The spots on this stock (MMRRC:000174) are sharply defined, which is not usual for Kit spotting.
MeSH Terms
  • Animals
  • Melanocytes/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains/genetics
  • Phenotype
  • Skin Pigmentation/genetics
  • Crosses, Genetic
  • Genes
  • Hair Color
  • Mice, Inbred C57BL/genetics
  • Mice, Mutant Strains/genetics
  • Base Sequence
  • Cell Differentiation/genetics
  • Cell Division/genetics
  • Cell Movement/genetics
  • DNA Primers/genetics
  • Enzyme Activation
  • Female
  • Gene Expression Regulation, Developmental
  • Genetic Markers
  • Lac Operon
  • Male
  • Melanocytes/cytology
  • Melanocytes/enzymology
  • Mice, Transgenic
  • Pigmentation Disorders/embryology
  • Pigmentation Disorders/genetics
  • Point Mutation
  • Proto-Oncogene Proteins c-kit/genetics
  • Proto-Oncogene Proteins c-kit/metabolism
  • Signal Transduction/genetics
  • Stem Cell Factor/genetics
  • Alleles
  • Chromosome Mapping
  • Embryonic and Fetal Development
  • Hematopoiesis
  • Mutation
  • Protein-Tyrosine Kinases/genetics
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins c-kit
  • Proto-Oncogenes
  • RNA, Messenger/analysis
  • Receptors, Cell Surface/genetics
Strain Development
Spontaneous mutation occurred on C57BL/6J. (MMRRC:000173) was selected for normal expression level of the Kit W-2J mutation compared to (MMRRC:000174) in which hi level expression was selected for and MMRRC:000175 in which low level expression of the mutation was selected for by continued inbreeding.
Suggested Control Mice
  • black littermates
  • C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Pigmentation
  • Developmental Biology
  • Gene regulation
  • Signaling molecules
  • Pattern formation

Strain Origin

Donor
Lynn Lamoreux, Ph.D., Texas A&M University
Primary Reference
  • Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Pigment. Cell Res. 1999 Dec;12(6):383-90. (Medline PMID: 10614578)
  • Lamoreux ML, Russell ES. Developmental interaction in the pigmentary system of mice. I. Interactions between effects of genes on color of pigment and on distribution of pigmentation in the coat of the house mouse (Mus musculus). J Hered. 1979 Jan-Feb;70(1):31-6. (Medline PMID: 469221)
  • Mackenzie MA, Jordan SA, Budd PS, Jackson IJ. Activation of the receptor tyrosine kinase Kit is required for the proliferation of melanoblasts in the mouse embryo. Dev Biol. 1997 Dec 1;192(1):99-107. (Medline PMID: 9405100)
  • Geissler EN, Ryan MA, Housman DE. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell. 1988 Oct 7;55(1):185-92. (Medline PMID: 2458842)

Colony and Husbandry Information

Special Considerations

Feed 9% fat breeder chow.

Kit heterozygous mice have fewer oocytes than normal mice and also the homozyotes are lethal in all cases, so spotted males should be mated to black females and they should be mated young. Each of these stocks is best maintained by intercrossing. If MMRRC:000175 is backcrossed to C57BL/6J the lo-spotting characterstic will be lost, but can be regained with inter-crossing. It is easier to mate inter se.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
See phenotype

Breeding

MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Heterozygous female x Heterozygous male
  • Wildtype female x Heterozygous male
  • Heterozygous female x Wildtype male
Generation
>F100
Overall Breeding Performance
Good

Reproductive Statistics

Average litter size
5
Recommended wean age
4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
000175-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit		 Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000175-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
000175-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.