Strain Name:
JU.B6-KitW-2J/aLmMmmh
Stock Number:
000177-MU
Citation ID:
RRID:MMRRC_000177-MU
Major Collection:

Strain Information

KitW-2J
Name: KIT proto-oncogene receptor tyrosine kinase; dominant spotting 2 Jackson
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Spontaneous Mutation
Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: c-KIT, belly-spot, CD117, SCO5, Tr-kit, SOW3, Steel Factor Receptor, Gsfsco1, Gsfsow3, Dominant white spotting, Gsfsco5, SCO1
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Spontaneous Mutation
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Genetic Alterations
Spontaneous mutation at Kit locus
Genotype Determination
Phenotype
Description of White Spotting mutants
Description of White Kit mutants

A dominant mutation that is lethal in the homozygous state. Mice that carry the mutant allele are black with white spotting; homozygous +/+ mice are solid black. Heterozygous mice with the mutant allele have fewer pigment cells (more white areas) than heterozygous mutant mice on the C57BL/J background.

Please note: This strain is identical to MMRRC:000178 in every aspect except that strain 178 has a high incidence of hypopigmention whereas 177 has a "normal" or average level of expression.

In this collection of mice, the identical allele of origin is expressed in five different degrees of severity (stocks 173, 174, 175, 177, 178), depending upon the congenic background, and within the congenic mice upon two (or more) discrete modifying genes that have been maintained by selection. This stock (MMRRC:000177) was created by backcrossing the original mutant, which was obtained from The Jackson Laboratory, where the mutation occurred in the C57BL/6J stocks of Dr. Elizabeth Russell, onto a JU/CtLm background. The allele is homozygous lethal. Heterozygotes exhibit at least twice as much white area as the same allele on the B6 background.
MeSH Terms
  • Animals
  • Crosses, Genetic
  • Genes
  • Hair Color
  • Mice
  • Mice, Inbred C57BL/genetics
  • Mice, Mutant Strains/genetics
  • Phenotype
  • Melanocytes/physiology
  • Mice, Inbred C57BL
  • Mice, Inbred Strains/genetics
  • Skin Pigmentation/genetics
  • Base Sequence
  • Cell Differentiation/genetics
  • Cell Division/genetics
  • Cell Movement/genetics
  • DNA Primers/genetics
  • Enzyme Activation
  • Female
  • Gene Expression Regulation, Developmental
  • Genetic Markers
  • Lac Operon
  • Male
  • Melanocytes/cytology
  • Melanocytes/enzymology
  • Mice, Transgenic
  • Pigmentation Disorders/embryology
  • Pigmentation Disorders/genetics
  • Point Mutation
  • Proto-Oncogene Proteins c-kit/genetics
  • Proto-Oncogene Proteins c-kit/metabolism
  • Signal Transduction/genetics
  • Stem Cell Factor/genetics
  • Alleles
  • Chromosome Mapping
  • Embryonic and Fetal Development
  • Hematopoiesis
  • Mutation
  • Protein-Tyrosine Kinases/genetics
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins c-kit
  • Proto-Oncogenes
  • RNA, Messenger/analysis
  • Receptors, Cell Surface/genetics
Strain Development
Spontaneous mutation arose on a C57BL/6J; subsequently backcrossed to JU/CtLm.
Suggested Control Mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Pigment cell precursors during embryogenesis
  • Gene interactions affecting pigmentation
  • Embryonic development of mast cells, germ cells or erythrocytes
Donor
Dr. ML Lamoreux, Texas A&M University
Primary Reference
  • Lamoreux ML, Russell ES. Developmental interaction in the pigmentary system of mice. I. Interactions between effects of genes on color of pigment and on distribution of pigmentation in the coat of the house mouse (Mus musculus). J Hered. 1979 Jan-Feb;70(1):31-6. (Medline PMID: 469221)
  • Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Pigment. Cell Res. 1999 Dec;12(6):383-90. (Medline PMID: 10614578)
  • Mackenzie MA, Jordan SA, Budd PS, Jackson IJ. Activation of the receptor tyrosine kinase Kit is required for the proliferation of melanoblasts in the mouse embryo. Dev Biol. 1997 Dec 1;192(1):99-107. (Medline PMID: 9405100)
  • Geissler EN, Ryan MA, Housman DE. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene. Cell. 1988 Oct 7;55(1):185-92. (Medline PMID: 2458842)
  • Lamoreux ML, Russell ES. Developmental interaction in the pigmentary system of mice. I. Interactions between effects of genes on color of pigment and on distribution of pigmentation in the coat of the house mouse (Mus musculus). J Hered. 1979 Jan-Feb;70(1):31-6. No abstract available. (Medline PMID: 469221)

Colony and Husbandry Information

Maintain these mice on a 9% fat breeder chow. Suggest use non-spotted JU/CtLm inbred females each generation if being maintained over a period of time. Kit heterozygous mice (KitW-2J/+) have fewer oocytes than normal mice and also the homozygotes are lethal in all cases, so spotted males should be mated to black females in all cases when possible. They should be mated when young. Each of these stocks is best maintained by intercrossing.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Heterozygotes are black with white spotting.
MMRRC Breeding System
Backcross or sib mating
Breeding Scheme(s)
  • JU/CtLm female x Heterozygote mutant male
  • Heterozygote mutant female x JU/CtLm male
  • Heterozygote mutant female x Heterozygote mutant male
Generation
Unknown
Overall Breeding Performance
Excellent
Average litter size
Unknown
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000177-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000177-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000177-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.