Strain Name:
JU.Cg-Ednrbs/Mmmh
Stock Number:
000180-MU
Citation ID:
RRID:MMRRC_000180-MU
Major Collection:

Strain Information

Ednrbs
Name: endothelin receptor type B; piebald
Synonyms: pied spotting, s
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Spontaneous Mutation
Ednrb
Name: endothelin receptor type B
Synonyms: Sox10m1, ETR-b, ETb
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Spontaneous Mutation
NCBI: 13618
VEGA: 14
HGNC: HGNC:3180
Homologene: 89
Genetic Alterations
This mutation is allelic to a targeted mutation of this gene. Homozygous mice produce approximately 25% of the normal levels of transcript from this allele. RT-PCR analysis demonstrated that no alterations in the coding sequence would result in any alteration of the amino acid sequence, and therefore could account for the mutant phenotype.
Genotype Determination
Phenotype
Heterozygous mice have a white stripe on the head and a prominant white belly spot. Homozygous mice are spotted and prone to the development of megacolon.
Mammalian Phenotype Terms
Allelic Composition: Ednrbs/Ednrbs (Genetic Background: Not Specified )

Allelic Composition: Ednrbs/Ednrbs-13Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-17FrS (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-17Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1Acrg (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1Chlc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1MLPf (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1Pu (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1XMLP (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-1XMLPc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-24Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-27Pu (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-27Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-29Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-2CHLd (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-2Etopc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-2MLPl (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-12MNURn (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs-15DttMb/Ednrbs (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-3CHLo (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-4Chlc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-4Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-36Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-31Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-3Etopf (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-48UThc (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-4Blec (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-52Pub (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbs/Ednrbs-9ThW (Genetic Background: involves: 101/Rl * C3H/Rl )

Allelic Composition: Ednrbtm1Ywa/Ednrbs (Genetic Background: involves: 129S/SvEv )

MeSH Terms
  • Animals
  • Melanocytes/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains/genetics
  • Phenotype
  • Skin Pigmentation/genetics
  • Base Sequence
  • Chromosome Mapping
  • Gene Deletion
  • Hair Color/genetics
  • Hirschsprung Disease/embryology
  • Hirschsprung Disease/genetics
  • Homozygote
  • Mice, Knockout
  • Mice, Mutant Strains
  • Molecular Sequence Data
  • Mutation
  • Neural Crest/growth & development
  • Receptor, Endothelin B
  • Receptors, Endothelin/genetics
Strain Development
Spontaneous mutation arose on "old mutant of the mouse fancy"; subsequently backcrossed to JU/CtLm (MMRRC:000136).
Suggested Control Mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Eye development
  • Megacolon research
  • Waardenburg syndrome type 4 research
  • Pigmentation
Donor
Dr. M. L. Lamoreux, Texas A&M University
Primary Reference
  • Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Pigment Cell Res. 1999 Dec;12(6):383-90. (Medline PMID: 10614578)
  • Hosoda K; Hammer RE; Richardson JA; Baynash AG; Cheung JC; Giaid A; Yanagisawa M. Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice. Cell. 1994 Dec 30;79(7):1267-76. (Medline PMID: 8001159)
  • Dunn LC, Mohr J. An association of hereditary eye defects with white spotting. Proc Natl Acad Sci U S A. 1952 (32): 872-875. (Medline PMC: 1063671)

Colony and Husbandry Information

Litters are larger than normal (10-12), so pair breeding is recommended if maintained in small cages. If maintained in larger cages, breeding trios are preferred, as two females care for the large litters better than one female.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Homozygous mice are black and white spotted. Heterozygous mice are black with white stripe on forehead.
MMRRC Breeding System
Sib mating or backcross
Breeding Scheme(s)
  • Heterozygous Female x Heterozygous Male
  • Heterozygous Female x JU/CtLm Male
  • JU/CtLm Female x Heterozygous Male
  • If homozygous mice survive, they can be used as breeders but it is recommended to mate homozygous mice to heterozygous mice due to the high mortality associated with the homozygous state.
Generation
N10+
Overall Breeding Performance
Excellent
Average litter size
10-12
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000180-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000180-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000180-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.