Strain Detail Sheet

Strain Name    :

JU.Cg-Ednrbs/Mmmh

Stock Number :

000180-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Ednrbs
Name: piebald
Chromosome: 14
Alteration at locus: Spontaneous Mutation
Gene Symbol: Ednrb
Name: endothelin receptor type B
Chromosome: 14
Alteration at locus: Spontaneous Mutation

Genetic Alterations:
This mutation is allelic to a targeted mutation of this gene. Homozygous mice produce approximately 25% of the normal levels of transcript from this allele. RT-PCR analysis demonstrated that no alterations in the coding sequence would result in any alteration of the amino acid sequence, and therefore could account for the mutant phenotype.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Heterozygous mice have a white stripe on the head and a prominant white belly spot. Homozygous mice are spotted and prone to the development of megacolon.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Ednrbs/Ednrbs
        Background Not Specified
  • pigmentation phenotype
    • abnormal foot pigmentation (MGI Ref ID J:30758)
    • abnormal tail pigmentation (MGI Ref ID J:30758)
    • variable depigmentation (MGI Ref ID J:30758)
    • white spotting (MGI Ref ID J:12952)
      • size of spots is variable depending on modifiers
      • variable body spotting (MGI Ref IDs J:12952, J:30758)
        • location and size of spots are variable depending on modifiers (MGI Ref ID J:12952)
        • background modifiers determine extent (MGI Ref ID J:30758)
        • predictable locations for spotting include the feet, tail, head, tip of nose, umbilical area, lumbar region and shoulders (MGI Ref ID J:30758)
        • this genotype may result in an entirely or almost entirely unpigmented phenotype or a mouse with few white spots of variable size (MGI Ref ID J:12952)
  • limbs/digits/tail phenotype
    • abnormal tail pigmentation (MGI Ref ID J:30758)
  • digestive/alimentary phenotype
    • megacolon (MGI Ref ID J:15014)
      • as many as 10% of any litter of homozygotes may exhibit m egacolon
  • integument phenotype
    • abnormal foot pigmentation (MGI Ref ID J:30758)
    • abnormal tail pigmentation (MGI Ref ID J:30758)
    • variable depigmentation (MGI Ref ID J:30758)
    • white spotting (MGI Ref ID J:12952)
      • size of spots is variable depending on modifiers
      • variable body spotting (MGI Ref IDs J:12952, J:30758)
        • location and size of spots are variable depending on modifiers (MGI Ref ID J:12952)
        • background modifiers determine extent (MGI Ref ID J:30758)
        • predictable locations for spotting include the feet, tail, head, tip of nose, umbilical area, lumbar region and shoulders (MGI Ref ID J:30758)
        • this genotype may result in an entirely or almost entirely unpigmented phenotype or a mouse with few white spots of variable size (MGI Ref ID J:12952)

Strain of Origin: old mutant of the mouse fancy

Strain genetic background: JU/CtLm (MMRRC:000136)

Strain Development: Spontaneous mutation arose on "old mutant of the mouse fancy"; subsequently backcrossed to JU/CtLm (MMRRC:000136).

Suggested Control Mice:

Research Applications

  • Eye development
  • Megacolon research
  • Waardenburg syndrome type 4 research
  • Pigmentation

Strain Origin

Donor: Dr. M. L. Lamoreux, Texas A&M University

Primary Reference:

  • Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Pigment Cell Res. 1999 Dec;12(6):383-90. (Medline: 10614578)
  • Hosoda K; Hammer RE; Richardson JA; Baynash AG; Cheung JC; Giaid A; Yanagisawa M. Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice. Cell. 1994 Dec 30;79(7):1267-76. (Medline: 8001159)
  • Dunn LC, Mohr J. An association of hereditary eye defects with white spotting. Proc Natl Acad Sci U S A. 1952 (32): 872-875. (Medline: Not available)

Colony and Husbandry Information

Special Considerations

Litters are larger than normal (10-12), so pair breeding is recommended if maintained in small cages. If maintained in larger cages, breeding trios are preferred, as two females care for the large litters better than one female.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Homozygous mice are black and white spotted. Heterozygous mice are black with white stripe on forehead.

Other:


Breeding

MMRRC Breeding System: Sib mating or backcross

Breeding Scheme(s):

  • Heterozygous Female x Heterozygous Male
  • Heterozygous Female x JU/CtLm Male
  • JU/CtLm Female x Heterozygous Male
  • If homozygous mice survive, they can be used as breeders but it is recommended to mate homozygous mice to heterozygous mice due to the high mortality associated with the homozygous state.

Generation: N10+

Overall Breeding Performance: Excellent

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Poor Poor
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: Unknown Unknown

Average litter size: 10-12

Recommended wean age: 4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000180-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000180-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
000180-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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