Strain Name:
B6J(Cg)-Adamts20bt-2H/Mmmh
Stock Number:
000183-MU
Citation ID:
RRID:MMRRC_000183-MU
Major Collection:

Strain Information

Adamts20bt-2H
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20; belted 2 Harwell
Synonyms: Adamts20bt-9J
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Spontaneous Mutation
Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: bt, ADAMTS-20
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Spontaneous Mutation
NCBI: 223838
Homologene: 11808
Genetic Alterations
Spontaneous mutation. The Adamts20bt-2H allele has a single C-to-T point mutation at nucleotide 2281, which is predicted to cause a missense mutation (Leu761Phe) in the spacer domain of Adamts20.

HGVS nomenclature:
  • Genbank RefSeq - mRNA: NM_177431.5
  • Genbank RefSeq, protein: NP_803180.3
  • Variant, nucleic acid level: c.2281C>T
  • Variant, amino acid level, predicted: p.Leu761Phe
    Check this variant: LUMC Mutalyzer
Genotype Determination
Phenotype
Description of White Spotting mutants

Homozygous mutants have a white belt across the back in the midtrunk region and a white belly patch that coalesces to form a white belt.

Belted is a recessive mutation that results in white-spotting. White-sptting is defined as the absence of pigmentation that is caused by absence of melanocytes, which is distinct from albinism in which the absence of pigment results from failure of melanogenesis within the pigment cells. The belted locus encodes "a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 20," that is evidently involved in survival of melanocytes. Homozygous mutants usually have a white belly patch and often a white belt across the back in the midtrucnk region. The two may coalesce to form a white belt. Two phenotypic characteristics distinguish belted mice from mice mutant at other white-spotting loci. First, the background genome does not seem to influence the location of the spot. Second pheomelanogenesis of the mouse has only a small influence upon the size of the spot.
Mammalian Phenotype Terms
Allelic Composition: Adamts20bt-2H/Adamts20bt-2H (Genetic Background: B6.Cg-Adamts20bt-2H )

Allelic Composition: Adamts20bt-2H/Adamts20bt-2H (Genetic Background: JU.Cg-Adamts20bt-2H )

MeSH Terms
  • Animals
  • Melanocytes/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains/genetics
  • Phenotype
  • Skin Pigmentation/genetics
  • ADAM Proteins
  • ADAMTS Proteins
  • Cell Movement/physiology
  • Disintegrins/chemistry
  • Disintegrins/classification
  • Disintegrins/genetics
  • Disintegrins/metabolism
  • Embryo, Mammalian/physiology
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • Metalloendopeptidases/chemistry
  • Metalloendopeptidases/classification
  • Metalloendopeptidases/genetics
  • Metalloendopeptidases/metabolism
  • Mutation
  • Phylogeny
  • Pigmentation/genetics
  • Skin/cytology
  • Skin/growth & development
  • Skin/metabolism
  • ADAM Proteins/genetics
  • ADAM Proteins/metabolism
  • Alleles
  • Base Sequence
  • Cell Differentiation
  • Cell Movement
  • Cell Proliferation
  • Cell Survival/genetics
  • Cell Survival/physiology
  • DNA Primers/genetics
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Female
  • Melanocytes/cytology
  • Melanocytes/enzymology
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Neural Crest/cytology
  • Neural Crest/embryology
  • Neural Crest/enzymology
  • Pregnancy
  • Proto-Oncogene Proteins c-kit/metabolism
  • Signal Transduction
  • Skin/embryology
  • Skin/enzymology
  • Skin Pigmentation/physiology
  • Versicans/metabolism
Strain Development
Mutant mice backcrossed to C57BL/6J
Suggested Control Mice

C57BL/6

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Pigment research
  • Developmental Biology
  • Gene Regulation
  • Signaling molecules
  • Cancer incidence
  • Obesity
  • Diabetes
  • Neural Crest Development
Donor
Lynn Lamoreux, Ph.D., Texas Agricultural and Mechanical University.
Primary Reference

Murray JM, Snell GD. Belted, a new sixth chromosome mutation in the mouse. J Hered 1945;36:266-8 (Medline: Not Available)

Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Pigment Cell Res. 1999 Dec;12(6):383-90. (Medline PMID: 10614578)

Rao C, Foernzler D, Loftus SK, Liu S, McPherson JD, Jungers KA, Apte SS, Pavan WJ, Beier DR. A defect in a novel ADAMTS family member is the cause of the belted white-spotting mutation. Development. 2003 Oct;130(19):4665-72. (Medline PMID: 12925592)

Silver DL, Hou L, Somerville R, Young ME, Apte SS, Pavan WJ. The secreted metalloprotease ADAMTS20 is required for melanoblast survival. PLoS Genet. 2008 Feb 29;4(2):e1000003. (Medline PMID: 18454205)

Colony and Husbandry Information

Feed 9% fat breeder chow.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black with white belt
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
Homozygous female x Homozygous male
Generation
N10+ (C57BL/6J), F10+
Overall Breeding Performance
Good
Average litter size
7
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000183-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000183-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.